We narrowed to 9,085 results for: mel

1. pXPR_sgFLNB-SK4

   Plasmid

   #119874

   Purpose

   gRNA expression vector

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   sgFLNB-SK4 (FLNB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GSK3beta (K85A)-pcw107-V5

   Plasmid

   #64614

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   GSK3B (GSK3B Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   K85A

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

3. AAV-CAG-FLPX-rc [Chronos-tdTomato]

   Plasmid

   #105834

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-Mbd3

   Plasmid

   #52356

   Purpose

   Lentiviral expression vector for constitutive expression of Mbd3

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-CASANOVA

   Plasmid

   #113036

   Purpose

   AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBJG1 T7-8xHis-OGT 5N5A

   Plasmid

   #154280

   Purpose

   Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations; bact…

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

   Plasmid

   #75118

   Purpose

   Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Mutation

   Bromodomain replaced with the bromodomain of BRD7

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pDECKO_GFP_AAVS1

   Plasmid

   #141211

   Purpose

   pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Insert

   gRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

   Plasmid

   #75116

   Purpose

   Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Mutation

   single amino acid mutation N216A

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pDECKO_mCherry_AAVS1_1801

    Plasmid

    #159090

    Purpose

    targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-BRD9_BET-PGK-Puro-IRES-GFP

    Plasmid

    #75120

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Mutation

    Bromodomain replaced with the first bromodomain o…

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

12. INCTbiosyn-p35S-GFP(rc)phiC31

    Plasmid

    #127527

    Purpose

    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. INCTbiosyn-p35S-GFP(rc)Bxb1

    Plasmid

    #127528

    Purpose

    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-dCTNNA1

    Plasmid

    #176032

    Purpose

    A knock-out vector for dog CTNNA1 (alpha-1-catenin)

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog CTNNA1 (alpha-1-catenin) gene and the cDNA of Cas9 (CTNNA1 )
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUW-CAGGS-ERAS

    Plasmid

    #52416

    Purpose

    Lentiviral vector encoding constitutive expression of human ERAS cDNA

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    ERAS (ERAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. BCL2-pcw107-V5

    Plasmid

    #64630

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    BCL2 (transcript variant alpha) (BCL2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA-DDX11-Flag-KAE

    Plasmid

    #120728

    Purpose

    Expression of a Flag-tagged version of DDX11 KAE mutant in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 KAE mutant (DDX11 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Substitution of E201 and Y202 with K and A

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1α1.1-Chronos-GFP

    Plasmid

    #122098

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1α (1.1 kb short version)

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-DDX11-Flag-KAK

    Plasmid

    #120729

    Purpose

    Expression of a Flag-tagged version of DDX11 KAK mutant in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 KAK mutant (DDX11 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Substitution of E201, Y202 and E203 with K, A and…

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223-ZNF698

    Plasmid

    #88666

    Purpose

    Donor Vector containing ZNF698 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF698 (ZFP57 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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