We narrowed to 10,047 results for: pET
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Plasmid#105787PurposeExpression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-MBPExpressionMammalianAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only
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pKK-TEV-EGFP
Plasmid#105793PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-EGFPExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mCherry-TEV
Plasmid#105779PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.DepositorTypeEmpty backboneUseFlp-in competentTagsmCherry-TEVExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mEGFP-TEV
Plasmid#105777PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).DepositorTypeEmpty backboneUseFlp-in competentTagsmEGFP-TEVExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-EGFP-TEV
Plasmid#105776PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)DepositorTypeEmpty backboneUseFlp-in competentTagsEGFP-TEVExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTcI39-switch
Plasmid#195557PurposeTemperature state switch expressing low-temperature mWasabi and high-temperature mRFP1DepositorInsertsmRFP1
mWasabi
TcI39
Tags6x His and AANDENYADAS degradation tagExpressionBacterialMutationA67TAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-Halo-10-PFN1
Plasmid#204222PurposeExpression of Halo-Profilin-1 in tissue culture cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPFN1 (PFN1 Human)
TagsHalo, TEV site, SGLRSRAQAS linkerExpressionMammalianPromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
BDD FVIII H3
Plasmid#46775PurposepcDNA4.BDD FVIII.H3(M2238V)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertB-domain deleted FVIII H3 protein (M2238V) (F8 Human)
ExpressionMammalianMutationM2238VPromoterCMVAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p53-(1-393)-E285K-N268D
Plasmid#35089DepositorInsertp53 (TP53 Human)
TagsHis and Thrombin cleavage siteExpressionBacterialMutationContains aa 1-393 with N269D and E285K mutationsPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
lenti-ATAD2-plx303
Plasmid#188635PurposeATAD2 overexpressionDepositorAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-H2B-GFP
Plasmid#184777PurposeExpresses H2B-GFP in human pluripotent stem cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2BC11 (H2BC11 Human)
TagsEGFPExpressionMammalianAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW-4146NES(ROCK)
Plasmid#162485PurposeFor generating transgenic mice expressing ROCK biosensor based on FRET.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertROCK biosensor based on FRET.
TagsYPet and ECFPExpressionMammalianPromoterCAGAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH652-RLuc/maxFLuc
Plasmid#29698DepositorInsertFirefly Luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…Available sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p11d-tRPA (MSW#111)
Plasmid#208066Purposeexpression of human Replication Protein A in E. coliDepositorExpressionBacterialMutationRPA32 has a point mutation Thr88(ACC) -> Ile(A…Available sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)
Plasmid#169079PurposeHuman ATG3DepositorInsertATG3 (ATG3 Human)
Tags6X Histidine Tag, TEV cleavage siteExpressionBacterialPromoterT7 lac promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p369_DG
Plasmid#234692PurposeGolden gate domesticated pET28b with 3C-His10DepositorTypeEmpty backboneTags3C-His10ExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p346_DG
Plasmid#234676PurposeGolden gate domesticated pET28b with His10-TEVDepositorTypeEmpty backboneTagsHis10-TEVExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p357_DG
Plasmid#234700PurposeGolden gate domesticated pET28b for scarless cloning with His10-GST-3CDepositorTypeEmpty backboneTagsHis10-GST-3CExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
U6+27-20
Plasmid#21977DepositorPublicationInsertU6 miR-20 sponge
ExpressionMammalianAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
PKA-N-HiBiT
Plasmid#211377PurposeBacterially expressed HiBiT-PKA for Ni-NTA purificationDepositorInsertHuman protein kinase cAMP-activated catalytic subunit alpha (PRKACA) transcript variant 1, with N-terminal HiBiT (PRKACA Human)
Tags10xHis-tag with thrombin site and HiBiT TagExpressionBacterialPromoterT7 PromotorAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only