We narrowed to 12,153 results for: 112

1. pEmpty_Control

   Plasmid

   #162607

   Purpose

   Empty control for Cas9 experiments and to clone new guide RNAs for paper

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Expression

   Yeast

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZJOM90

   Plasmid

   #133647

   Purpose

   Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pTPI1-SS-mCherry-HDEL
   

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

   Plasmid

   #126553

   Purpose

   Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

   Depositor

   Jonathan Karn

   Article

   Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

   Insert

   HIV-1 vector pNL4-3
   

   Use

   Lentiviral

   Tags

   Thy1.2 and GFP

   Promoter

   HIV LTR

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0052-REPAIR non-targeting guide clone into pC0043

   Plasmid

   #103868

   Purpose

   Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   non-targeting guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCasKP-hph

   Plasmid

   #117232

   Purpose

   Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUB_smFLAG_KDM5B_MS2

   Plasmid

   #81084

   Purpose

   Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smFLAG_KDM5B_24xMS2
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDAI-SceI-pheS

   Plasmid

   #111611

   Purpose

   Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Insert

   pheS
   

   Expression

   Bacterial

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CT452

    Plasmid

    #233665

    Purpose

    Red light-inducible Flp-N; Flp-N396-FHY1

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    Flp-N396-FHY1
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. CT438

    Plasmid

    #233666

    Purpose

    Red light-inducible Flp-N; Flp-N27-PhyA

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    Flp-N27-PhyA
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CAG

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. CT445

    Plasmid

    #233668

    Purpose

    Red light-inducible Flp-C; Flp-397C-PhyA

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    Flp-397C-PhyA
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CT447

    Plasmid

    #233663

    Purpose

    Red light-inducible Flp-C; Flp-28C-FHY1

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    Flp-28C-FHY1
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CAG

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. ALIX-mNeonGreen

    Plasmid

    #191191

    Purpose

    Full-length ALIX with C-terminal mNeonGreen tag.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (PDCD6IP Human, Synthetic)
    

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPP381

    Plasmid

    #158798

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-2
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    RuvC-inactivating mutation D394A

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. XPO6-EGFP

    Plasmid

    #210371

    Purpose

    Expresses XPO6 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    XPO6 (XPO6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRel-Hyg

    Plasmid

    #227582

    Purpose

    Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCdrA-gfpC

    Plasmid

    #111614

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pADH100

    Plasmid

    #90980

    Purpose

    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. NLRP3-mNeonGreen

    Plasmid

    #200942

    Purpose

    NLRP3 fused with mNeonGreen to indicate its localization change

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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