We narrowed to 9,975 results for: Uty

1. psLIB EF1alpha

   Plasmid

   #229954

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. psLIB TRE

   Plasmid

   #229955

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA19_sgRNA-1_M13ps

   Plasmid

   #231409

   Purpose

   M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Insert

   sgRNA-1
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE-9-RTXWTM-X2S-C

   Plasmid

   #225967

   Purpose

   Block V Repeats-In-Toxin (RTX) protein with global substitution of serine in nonapeptide repeats and C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXWTM-X2S-C
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE-9-RTXWTM-X2N-C

   Plasmid

   #225968

   Purpose

   Block V Repeats-In-Toxin (RTX) protein with global substitution of asparagine in nonapeptide repeats and C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXWTM-X2N-C
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE-9-RTXWTM-X2D-C

   Plasmid

   #225969

   Purpose

   Block V Repeats-In-Toxin (RTX) protein with global substitution of aspartic acid in nonapeptide repeats and C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXWTM-X2D-C
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE-9-RTXWTM-X2E-C

   Plasmid

   #225970

   Purpose

   Block V Repeats-In-Toxin (RTX) protein with global substitution of glutamic acid in nonapeptide repeats and C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXWTM-X2E-C
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQE-9-RTXWTM-X2A-C

   Plasmid

   #225965

   Purpose

   Block V Repeats-In-Toxin (RTX) protein with global substitution of alanine in nonapeptide repeats and C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXWTM-X2A-C
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE-9-RTXWTM-X2H-C

   Plasmid

   #225966

   Purpose

   Block V Repeats-In-Toxin (RTX) protein with global substitution of histidine in nonapeptide repeats and C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXWTM-X2H-C
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rab11A[8-175]-His-C Q70L

    Plasmid

    #228042

    Purpose

    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).

    Depositor

    Wade Harper

    Insert

    Rab11A[8-175]-His-C Q70L (RAB11A Human)
    

    Expression

    Bacterial

    Mutation

    Q70L

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBH504

    Plasmid

    #215782

    Purpose

    NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene

    Depositor

    Corey Wilson

    Article

    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

    Inserts

    LacI(YQR)
    sgRNA-ribozyme cassette
    dCas9
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Bt1311, P(O1), and P1

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. L1P27

    Plasmid

    #195929

    Purpose

    Level 1 PhiC31 cassette with constitutive promoter: p35S-UbArg-PhiC31NLS-tUBQ1

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    p35S-UbArg-PhiC31NLS-tUBQ1
    

    Expression

    Plant

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPCTαKN0001

    Plasmid

    #187568

    Purpose

    Transcriptional unit for constitutive expression of firefly luciferase driven by the 35s promoter

    Depositor

    Nicola Patron

    Article

    Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

    Insert

    Luciferase
    

    Expression

    Plant

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GB UA_114 A

    Plasmid

    #187570

    Purpose

    Transcriptional unit for constitutive expression of Gal4:ΦC31 driven by the 35s promoter

    Depositor

    Nicola Patron

    Article

    Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

    Insert

    Gal4:ΦC31
    

    Expression

    Plant

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEPKKα1KN0253

    Plasmid

    #187560

    Purpose

    Transcriptional unit for constitutive expression of CUP2:GAL4 driven by the nos promoter

    Depositor

    Nicola Patron

    Article

    Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

    Insert

    CUP2:GAL4
    

    Expression

    Plant

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL4624 (pEffector_TnpB1,ωRNA)

    Plasmid

    #210870

    Purpose

    Expresses GstTnpB1 and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstTnpB1
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

    Plasmid

    #204724

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. L1P4

    Plasmid

    #195919

    Purpose

    Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-DST-tUBQ1

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pPP2AA3-PhiC31-DST-tUBQ1
    

    Expression

    Plant

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. L1P2

    Plasmid

    #195918

    Purpose

    Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-tUBQ1

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pPP2AA3-PhiC31-NLS-tUBQ1
    

    Expression

    Plant

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. L1P16

    Plasmid

    #195921

    Purpose

    Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-tUBQ1

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pUBQ10-PhiC31-tUBQ1
    

    Expression

    Plant

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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