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MTK3_037

Plasmid

#123751

Purpose

Encodes cAMPr, a cAMP reporter, as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

cAMPr

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r85

Plasmid

#133414

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 85

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r78 (Vmn1r78 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP5g-P2A-Vmn1r89

Plasmid

#133416

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 89

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry-Vmn1r89 (Vmn1r89 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAG_ToeRep_N09_switch

Plasmid

#132741

Purpose

T7 RNAP-driven expression of first-generation toehold repressor index 9 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRep_N09_switch

Expression

Bacterial

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N19_switch

Plasmid

#132744

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 19 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N19_switch

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N64_switch

Plasmid

#132743

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 64 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N64_switch

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJPL3[flp-6p::cmk-1::GFP]

Plasmid

#124613

Purpose

Used to confirm the expression of the trans-spliced GFP reporter in the expected ASE neurons

Depositor

Anne Brunet

Article

Lim et al J Neurosci. 2018 Jul 4;38(27):6114-6129.

Insert

[flp-6p::cmk-1::GFP]

Expression

Worm

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGC684

Plasmid

#107022

Purpose

GFP reporter of lag-2 driven by 1 kb of promoter, which lackes both DBS and Cons motifs

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Tags

GFP

Expression

Worm

Promoter

lag-2

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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LOC5570372 NPFF receptor

Plasmid

#115378

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5570372 NPFF receptor (LOC5570372 Aedes aegypti)

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-PACT 5'-UTR

Plasmid

#115351

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of PACT

Depositor

Ming-Chih Lai

Article

Lai et al FEBS J. 2016 Jan;283(1):88-101. doi: 10.

Insert

PACT 5'-UTR (PRKRA Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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ACS_4

Plasmid

#111646

Purpose

Funclib design ACS_4

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_4

Tags

6xHis

Expression

Bacterial

Mutation

see depositor comments below

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Cul1b-3'UTR

Plasmid

#97153

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Cul1b 3'UTR

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Traf6-3'UTR

Plasmid

#97154

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Traf6 3'UTR

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSi-check2-zRac2 - mut 3'UTR

Plasmid

#97159

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

Dre-Rac2 3'UTR

Expression

Mammalian

Mutation

miR-722 Binding Mutant

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSi-check2-zRac2 3'UTR

Plasmid

#97158

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

Dre-Rac2 3'UTR

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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TF101-STTM-miR394

Plasmid

#84813

Purpose

Turning off/down miR394 in Soybean

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM-miR394

Expression

Plant

Mutation

bp8 is deleted- please see depositor comments bel…

Promoter

unknown

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPB6

Plasmid

#83557

Purpose

Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL10 promoter-yEGFP

Expression

Yeast

Promoter

GAL10

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBRN168-MXMT

Plasmid

#79819

Purpose

DsREDmonomer-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

RN168/DsREDmonomer (1–168)

Expression

Plant

Promoter

CaMV 35S promoter and CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode9

Plasmid

#83686

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode9

Use

Retroviral

Mutation

please view depositor comments below

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch5_155183064-gRNA-rev

Plasmid

#81215

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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