We narrowed to 146,921 results for: ins;

1. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

   Plasmid

   #208953

   Purpose

   A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

   Plasmid

   #208952

   Purpose

   A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-TEV-mRuby3

   Plasmid

   #105797

   Purpose

   Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM33_Nsp1

   Plasmid

   #156465

   Purpose

   Bacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

   Plasmid

   #174633

   Purpose

   Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Use

   Tags

   mCherry-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM33_N_CTD

   Plasmid

   #156484

   Purpose

   Bacterial expression of Sars-CoV2 N_CTD protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   N_CTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM33_N_NTD

   Plasmid

   #156483

   Purpose

   Bacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   N_NTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. BAF-WT-tet-puro

   Plasmid

   #199648

   Purpose

   Doxycycline-inducible BAF expression

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   BAF (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MARK3_HUMAN_D0

   Plasmid

   #79740

   Purpose

   This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MARK3 (MARK3 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid

    #208944

    Purpose

    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-SLC10A4_STOP

    Plasmid

    #161109

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC10A4 (SLC10A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pKK-TEV-mCherry

    Plasmid

    #105796

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KCC2G_HUMAN_D0

    Plasmid

    #79711

    Purpose

    This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC2G (CAMK2G Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pDONR221-SPNS2_STOP

    Plasmid

    #161473

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SPNS2 (SPNS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. p-mCherry-C1-Aurora_CA

    Plasmid

    #98170

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX Abl-SH2

    Plasmid

    #46402

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Abl (ABL1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 120-225

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. EPHB1_HUMAN_D0

    Plasmid

    #79694

    Purpose

    This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    EPHB1 (EPHB1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP

    Plasmid

    #221893

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    TAF15 (TAF15 Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-mGFP

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-SFXN2_STOP

    Plasmid

    #161422

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SFXN2 (SFXN2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. STK3_HUMAN_D0

    Plasmid

    #79689

    Purpose

    This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK3 (STK3 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only