We narrowed to 8,680 results for: sgRNA

1. pSCL452

   Plasmid

   #219502

   Purpose

   Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

   Insert

   GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
   

   Expression

   Yeast

   Promoter

   GAL7

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

   Plasmid

   #192131

   Purpose

   Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDe_Cas9_KANA_R160a

   Plasmid

   #173756

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/R160a_sgRNA2/R160a
   

   Use

   CRISPR

   Promoter

   AtU6 &StU6

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDe_Cas9_KANA_R160b

   Plasmid

   #173757

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/R160b_sgRNA2/160b
   

   Use

   CRISPR

   Promoter

   AtU6 &StU6

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDe_Cas9_KANA_R390a

   Plasmid

   #173758

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/390a_sgRNA2/390a
   

   Use

   CRISPR

   Promoter

   AtU6 &StU6

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. dora[B]_rescue_construct

   Plasmid

   #190637

   Purpose

   Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Expression

   Insect

   Mutation

   Changed threonine 295 to methionine

   Promoter

   Actin-5c

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. dora[A]_rescue_construct

   Plasmid

   #190639

   Purpose

   Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Expression

   Insect

   Mutation

   Truncated to contain amino acids 1-945

   Promoter

   Actin-5c

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPQD0CM0033

   Plasmid

   #185624

   Purpose

   Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-CTCG

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   NbU6-2
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPQD0CM0032

   Plasmid

   #185623

   Purpose

   Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCG

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   NbU6-1
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK434

    Plasmid

    #155378

    Purpose

    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK422

    Plasmid

    #155373

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK423

    Plasmid

    #155374

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458-Lck

    Plasmid

    #159430

    Purpose

    CRISPR knockout of Lck

    Depositor

    Arthur Salomon

    Article

    Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

    Insert

    sgRNA targeting Lck exon 2 (LCK Human)
    

    Use

    CRISPR

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKM328

    Plasmid

    #134243

    Purpose

    Mammalian expression construct expressing sgRNA against PLK1

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    gRNA targeting PLK1 3'
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA0057

    Plasmid

    #131784

    Purpose

    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer GFP cassette guide (gGFP) and 5' sgRNA
    

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLK4 G8.1 gRNA

    Plasmid

    #90837

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK4

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PLK4 (Guide Designation G8.1)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PLK4 G7.1 gRNA

    Plasmid

    #90836

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK4

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PLK4 (Guide Designation G7.1)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MIS18BP1 F10.1 gRNA

    Plasmid

    #90769

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MIS18BP1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MIS18BP1 (Guide Designation F10.1)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MCM6 E1.2 gRNA

    Plasmid

    #90762

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MCM6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MCM6 (Guide Designation E1.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MASTL F4.1 gRNA

    Plasmid

    #90753

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MASTL

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MASTL (Guide Designation F4.1)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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