We narrowed to 10,419 results for: plasmids 101

1. pCMV6-AN-DDK_V222A RAD23B

   Plasmid

   #201580

   Purpose

   Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutation V222A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV6-AN-DDK_F400A RAD23B

   Plasmid

   #201581

   Purpose

   Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutation F400A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TERTp124_Gluc_CCR5

   Plasmid

   #192271

   Purpose

   Donor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporter

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   Promoter Reporter Insert within donor template
   

   Use

   CRISPR

   Mutation

   -124 C to T

   Promoter

   TERT promoter driving expression of gaussia

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TERTp146_Gluc_CCR5

   Plasmid

   #192272

   Purpose

   Donor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporter

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   Promoter Reporter Insert within donor template
   

   Use

   CRISPR

   Mutation

   -146 C to T

   Promoter

   TERT promoter driving expression of gaussia

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PB-TAC-OK+9MS-KRAB-dCas9

   Plasmid

   #120357

   Purpose

   piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OK+9MS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [1-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-EGxxFP-PIGA

   Plasmid

   #153958

   Purpose

   Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   PIGA (a 401-bp fragment from intron 5 and exon 6)
   

   Use

   A plasmid specific for egxxfp assay

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CAG promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLM033

   Plasmid

   #134245

   Purpose

   Retroviral plasmid with H4 EGFP insert

   Depositor

   Iain Cheeseman

   Article

   Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

   Insert

   Human H4 + eGFP/HALO retroviral tagging (HRH4 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LMX1B-gRNA-C-del

   Plasmid

   #131516

   Purpose

   gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   LMX1B-gRNA-C-del
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

9. pK_lacO-cP-AOX1-AtX+NpgA

   Plasmid

   #126727

   Purpose

   yeast plasmid overexpressing AtX and NpgA

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   atX,npgA
   

   Tags

   6xHis

   Expression

   Yeast

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV2-U6gRNA5(Zfp219-g1)-PGKpuroBFP-W

    Plasmid

    #105021

    Purpose

    Lentiviral gRNA plasmid targeting mouse Zfp219 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Zfp219 (Zfp219 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2-U6gRNA5(Ndufa2-g1)-PGKpuroBFP-W

    Plasmid

    #105030

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ndufa2 (Ndufa2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W

    Plasmid

    #105031

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ndufa2 (Ndufa2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6gRNA5(Vps25-g1)-PGKpuroBFP-W

    Plasmid

    #105033

    Purpose

    Lentiviral gRNA plasmid targeting mouse Vps25 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Vps25 (Vps25 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKLV2-U6gRNA5(Tcf7l1-g2)-PGKpuroBFP-W

    Plasmid

    #105016

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tcf7l1 (Tcf7l1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKdH_P-GAP-ScADH2

    Plasmid

    #126734

    Purpose

    yeast plasmid overexpressing ADH2 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pK_-P-GAP-NpgA+AtX

    Plasmid

    #126725

    Purpose

    yeast plasmid overexpressing AtX and NpgA

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    atX,npgA
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG

    Plasmid

    #127325

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG

    Plasmid

    #127324

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG

    Plasmid

    #127327

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG

    Plasmid

    #127326

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge