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KG#493

Plasmid

#110932

Purpose

Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

Depositor

Kenneth Miller

Article

Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

Inserts

unc-17 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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KG#94

Plasmid

#110931

Purpose

Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

Depositor

Kenneth Miller

Article

Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

Inserts

unc-17 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 C204S

Plasmid

#111782

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C204S

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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PatoK-OG241

Plasmid

#72840

Purpose

NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoK

Expression

Bacterial

Promoter

PatoK

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PatoL-LOG241

Plasmid

#72846

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoL

Expression

Bacterial

Promoter

PatoL

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PatoK-LOG241

Plasmid

#72848

Purpose

NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoK

Expression

Bacterial

Promoter

PatoK

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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LLhT-pHG165a V52A

Plasmid

#90050

Purpose

Constitutive expression of a chimeric LacI:TreR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhT/V52A

Expression

Bacterial

Mutation

V52A

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhA-pHG165a Q55A

Plasmid

#90052

Purpose

Constitutive expression of a chimeric LacI:AscG transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhA/Q55A

Expression

Bacterial

Mutation

Q55A

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhC-pHG165a Q55I

Plasmid

#90053

Purpose

Constitutive expression of a chimeric LacI:CytR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhC/Q55I

Expression

Bacterial

Mutation

Q55I

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMT2 Cav2.2 DomI and DomII

Plasmid

#89891

Purpose

Mammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

Cacna1b (CACNA1B )

Expression

Mammalian

Mutation

Stop codon after amino acid 1154 (this is a trunc…

Promoter

adenovirus major late promoter

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-a4-delta tail

Plasmid

#80018

Purpose

Mammalian expression of human integrin alpha4 with no tail

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Expression

Mammalian

Mutation

no cytoplasmic tail (amino acids 1 to 1007)

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3H09

Plasmid

#71003

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

SNF4Agamma (SNF4Agamma Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4F04

Plasmid

#71030

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Sin3A (Sin3A Fly)

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2B09

Plasmid

#70918

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

gro (gro Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2C08

Plasmid

#70911

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf5 (Taf5 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C03

Plasmid

#70959

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf4 (Taf4 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2C05

Plasmid

#70891

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc2 (Orc2 Fly)

Use

Gateway entry vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
pET30Xa-SBL1(160E)

Plasmid

#66846

Purpose

Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)

Tags

His-tag

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11062

Plasmid

#68256

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-2

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pET30-SBL1(160S,HisTagDel)

Plasmid

#66880

Purpose

Expresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160S)

Tags

none

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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