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pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

Plasmid

#174029

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-mScarlet

Use

Zebrafish knock-in tagging

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTR-340 pUC19-tNGFR-P2A-IL2RG N

Plasmid

#186067

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

IL2RG (IL2RG Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

Plasmid

#114088

Purpose

U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for MbCas12a crRNAs

Expression

Mammalian

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ORF8-3xFLAG

Plasmid

#253138

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), 3xFLAG C-terminal of orf8

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_ORF8-3xFLAG

Tags

3X FLAG

Expression

Bacterial

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ORF7b-3xFLAG

Plasmid

#253137

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), 3xFLAG C-terminal of orf7b

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_ORF7b-3xFLAG (ORF7b SARS-CoV-2)

Tags

3X FLAG

Expression

Bacterial

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_3xFLAG-ORF7a

Plasmid

#253136

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), 3xFLAG N-terminal of orf7a

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_3xFLAG-ORF7a (ORF7a SARS-CoV-2)

Tags

3X FLAG

Expression

Bacterial

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2 frD_ΔrE/GFP

Plasmid

#253120

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope (residual AA). GFP is inserted in the locus of envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D2 SARS-CoV-2 ΔE-GFP

Expression

Bacterial

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-PolB-Arms1

Plasmid

#187197

Purpose

POLB gene homologous arm fragments to tag the POLB gene.

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2026 Apr 13;54(7):gka

Insert

Fragments of the PolB gene (POLB Human)

Use

Cloning vector

Promoter

LacZa

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD ΔS/GFP

Plasmid

#253144

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of spike, GFP is inserted at the locus of Spike

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD ΔS/GFP

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ORF6-BirA

Plasmid

#253132

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), BirA fused to orf6, C-terminal

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_ORF6-BirA (ORF6 SARS-CoV-2)

Tags

BirA

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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