We narrowed to 2,854 results for: 623

1. pRK5_msfGFP-HMGB1-Mut-F12A

   Plasmid

   #237623

   Purpose

   For overexpression of msfGFP-HMGB1-Mut-F12A

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   HMGB1-Mut-F12A (HMGB1 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   F12A

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiGuide-chr20-49345720-gRNA1

   Plasmid

   #232623

   Purpose

   Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

   Depositor

   Claes Wadelius

   Article

   Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

   Insert

   S. pyogenes sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

   Plasmid

   #176236

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176237

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176238

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-ZNF879

   Plasmid

   #101623

   Purpose

   Donor Vector containing ZNF879 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF879 (ZNF879 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LSB-hsa-miR-543

   Plasmid

   #103623

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-543 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-543 target (MIR543 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

   Plasmid

   #162379

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8f
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   Synapsin

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

   Plasmid

   #162380

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   CAG

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

    Plasmid

    #236231

    Purpose

    AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162378

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

    Plasmid

    #162377

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid

    #86233

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE

    Plasmid

    #162382

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    CAG

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162381

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    CAG

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV hSyn INTRON mScarlet-CAAX2 WPRE

    Plasmid

    #236232

    Purpose

    AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mScarletI fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mScarletI

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Notch3 intracellular domain-pcw107-V5

    Plasmid

    #64623

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    NOTCH3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    intracellular domain *

    Promoter

    PGK

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA.3.1.ADRB2-NP

    Plasmid

    #134376

    Purpose

    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    ADRB2-NP (ADRB2 Human)
    

    Use

    Tags

    Flag, natural peptide of nanoluciferase, and sign…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFl Strep-sumo-TEV-hAgo2 D597N

    Plasmid

    #136236

    Purpose

    Expressing catalytically inactive human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Use

    Tags

    Sterp Sumo

    Expression

    Insect

    Mutation

    D597N

    Promoter

    polyhedrin

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-2xFLAG-2xSTREP-CCND3

    Plasmid

    #172623

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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