We narrowed to 1,221 results for: POLI

1. pET28_6xHis-PARP1-A870L

   Plasmid

   #174797

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A870L (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A870L, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1-K893I

   Plasmid

   #174799

   Purpose

   Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-K893I (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K893I, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNSA-FLUC

   Plasmid

   #98143

   Purpose

   Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, zeocin resistance cassette

   Depositor

   Eva Farre

   Article

   Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   HA

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNOC-RLUC

   Plasmid

   #98142

   Purpose

   Gateway compatible construct for N' terminal fusion or promoter driven renilla luciferase reporter, hygromycin resistance cassette

   Depositor

   Eva Farre

   Article

   Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   HA

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNOC-NLUC

   Plasmid

   #98141

   Purpose

   Gateway compatible construct for N' terminal fusion or promoter driven NanoLuciferase reporter, hygromycin resistance cassette

   Depositor

   Eva Farre

   Article

   Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   HA

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNOC-FLUC

   Plasmid

   #98140

   Purpose

   Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, hygromycin resistance cassette

   Depositor

   Eva Farre

   Article

   Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   HA

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNOC-stacked-GeNL

   Plasmid

   #101002

   Purpose

   Construct for fusion expression of GeNL reporter and neomycin/G418 resistance gene by a bidirectional promoter

   Depositor

   Eva Farre

   Article

   Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Promoter

   Ribi

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHnCB

   Plasmid

   #52064

   Purpose

   expresses nine genes from the H. neapolitanus carboxysome operon; IPTG-inducible

   Depositor

   David Savage

   Article

   Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

   Insert

   CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B
   

   Expression

   Bacterial

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB_TRE3G_dCas9-5xGCN4_Hygro

   Plasmid

   #235572

   Purpose

   Dox-inducible expression of dCas9-GCN4 & genomic integration

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   dCas9-5xGCN4
   

   Use

   CRISPR

   Mutation

   D10A; H840A

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB_TRE3G_scFV-sfGFP

    Plasmid

    #235573

    Purpose

    Dox-inducible expression of control superfolder (sf)GFP fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    superfolder (sf)fGFP
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHnCBS1D

    Plasmid

    #52065

    Purpose

    expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible

    Depositor

    David Savage

    Article

    Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

    Insert

    CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
    

    Expression

    Bacterial

    Available Since

    April 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC-stacked-GFP-aequorin

    Plasmid

    #101011

    Purpose

    Construct for expression of a calcium sensitive luciferase reporter, GFP-aequorin, and hygromycin resistance gene by a bidirectional promoter

    Depositor

    Eva Farre

    Article

    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Promoter

    Ribi

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNOC-ARS-stacked-NeoR-mNeonGreen

    Plasmid

    #101249

    Purpose

    Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

    Depositor

    Eva Farre

    Article

    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Promoter

    Ribi

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNOC-stacked-aequorin

    Plasmid

    #101010

    Purpose

    Construct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoter

    Depositor

    Eva Farre

    Article

    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Promoter

    Ribi

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pet11a-ABLE

    Plasmid

    #158627

    Purpose

    Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

    Depositor

    William DeGrado

    Article

    Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

    Insert

    Apixaban-Binding Helical Bundle
    

    Tags

    6x His-tag and TEV cleavage tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBz118_His_CsoS2

    Plasmid

    #140841

    Purpose

    Halothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNOC-stacked-GeNL-Ca

    Plasmid

    #101003

    Purpose

    Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

    Depositor

    Eva Farre

    Article

    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB_mU6_enh-gRNA_Puro-T2A-BFP

    Plasmid

    #235571

    Purpose

    Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    sgRNA backbone
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLz37_CB_operon_CsoS2_N1-N4

    Plasmid

    #140856

    Purpose

    Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Expression

    Bacterial

    Available Since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBz15_Strep_Rubisco

    Plasmid

    #140839

    Purpose

    wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    Form IA Rubisco; CbbL, CbbS
    

    Tags

    Strep II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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