We narrowed to 1,224 results for: POLI

1. pMAH-POLY

   Plasmid

   #64915

   Purpose

   Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

   Inserts

   Polyspecific aminoacyl-tRNA Synthetase
   Amber Suppression tRNA
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6/H1

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEP PolI

   Plasmid

   #11722

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I EP (polA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D424A, I709N, A759R. Error-prone version of PolI.

   Promoter

   Available since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWT PolI

   Plasmid

   #11721

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I (polA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   wild-type

   Promoter

   Available since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7CFE1_polio_3ABC

   Plasmid

   #212986

   Purpose

   Encodes Poliovirus type I Mahoney 3ABC optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Poliovirus Type I Mahoney 3ABC
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPolI-PA-66

   Plasmid

   #240810

   Purpose

   Expresses influenza A virus mvRNA PA-66

   Depositor

   AJ te Velthuis

   Insert

   mvRNA PA-66
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPolI-PA-60

   Plasmid

   #240809

   Purpose

   Expresses influenza A virus mvRNA PA-60

   Depositor

   AJ te Velthuis

   Insert

   mvRNA PA-60
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pol I

   Available since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOliSpec5'

   Plasmid

   #228551

   Purpose

   Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 5' region of aadA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOliSpec3'

   Plasmid

   #228550

   Purpose

   Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

   Depositor

   Jeferson Gross

   Insert

   Truncated 3' region of aadA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7CFE1_polio_3ABC_C147R

   Plasmid

   #212985

   Purpose

   Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Poliovirus Type I Mahoney 3ABC C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   cysteine 147 in 3C changed to arginine

   Promoter

   T7

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7CFE1_polio_VP12A

    Plasmid

    #212988

    Purpose

    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus type I Mahoney VP12A
    

    Use

    Mammalian cell free protein expression

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7CFE1_polio_VP12A_C109R

    Plasmid

    #212987

    Purpose

    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus Type I Mahoney VP12A C109R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Expression

    Mutation

    Cysteine 109 from 2A mutated to arginine

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PoliIFN-γ

    Plasmid

    #107289

    Purpose

    Plasmid can be used for secretion of fish Interferon gamma

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

    Insert

    Interferon gamma
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPolII-Luc

    Plasmid

    #17223

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    PolII promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pPolI-NP71.2_inverted

    Plasmid

    #240808

    Purpose

    Expresses influenza A virus synthetic mvRNA NP71.2

    Depositor

    AJ te Velthuis

    Insert

    mvRNA NP71.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pol I

    Available since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPolI-NP71.6

    Plasmid

    #240813

    Purpose

    Expresses influenza A virus synthetic mvRNA NP71.6

    Depositor

    AJ te Velthuis

    Insert

    mvRNA NP71.6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pol I

    Available since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPolI-NP71.1

    Plasmid

    #240807

    Purpose

    Expresses influenza A virus synthetic mvRNA NP71.1

    Depositor

    AJ te Velthuis

    Insert

    NP71.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber, David Schaffer

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPolI_SARS-CoV-2_NLuc

    Plasmid

    #183953

    Purpose

    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

    Depositor

    AJ te Velthuis

    Article

    Vial et al bioRxiv 2021.06.28.450211

    Insert

    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mammalian Pol I

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV6-AN-DDK_WT POLI

    Plasmid

    #131228

    Purpose

    Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Coding sequence has been codon optimised for expr…

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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