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Plasmid#117531PurposeLevel0 Golden Gate part: CDS, SaCas9DepositorInsertSaCas9 (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 WWP1
Plasmid#67125PurposeStandard for cell-free expression benchmarking.DepositorInsertWWP1 (WWP1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G02
Plasmid#67136PurposeGateway destination vector for cell-free expression with C-term 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsHisExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJEP217-Lenti-CMV-GFP-WPRE-pA
Plasmid#62925PurposepLenti vector backbone designed to express GFP from a CMV promoter.DepositorInsertGreen Fluorescent protein (GFP)
UseLentiviralTagsExpressionMutationPromoter1.3αCaMKII and CMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 POLD1
Plasmid#67128PurposeStandard for cell-free expression benchmarking.DepositorInsertPOLD1 (POLD1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 RAD54B
Plasmid#67124PurposeStandard for cell-free expression benchmarking.DepositorInsertRAD54B (RAD54B Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFAP2B
Plasmid#67092PurposeStandard for cell-free expression benchmarking.DepositorInsertTFAP2B (TFAP2B Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ 3xFLAG-TREX1
Plasmid#164243Purposeretroviral plasmid for 3xFLAG-TREX1DepositorInsertTREX1 (TREX1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-PTP-NEO
Plasmid#51710Purposecontains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)ExpressionMutationPromoterAvailable sinceMarch 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pr-deGFP
Plasmid#67743PurposePr promoter expressing deGFPDepositorInsertUTR1-deGFP-T500
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLC-BB1
Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100 constitutiveAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorInsertSpCas9-p (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP204-Lenti-TRE3G-GFP-WPRE-Pa
Plasmid#164788PurposepLenti vector backbone designed to express GFP from a TRE3G promoter.DepositorInsertGreen Fluorescent protein (GFP)
UseLentiviralTagsExpressionMutationPromoter1.3αCaMKIIAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-2xFRB
Plasmid#20224DepositorInsert2xFRB (MTOR Human)
UseTagsHA and VhExpressionMammalianMutationOne copy of FRB has a T2098L mutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pN-PURO-PTP
Plasmid#51711Purposecontains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)ExpressionMutationPromoterAvailable sinceMarch 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP (pJJF001)
Plasmid#173457PurposeC-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJEP203-Lenti-TRE3G-GFPGluN2B-WPRE-pA
Plasmid#164787PurposepLenti vector backbone designed to express GFP-GluN2B from a TRE3G promoter.DepositorInsertGluN2B
UseLentiviralTagsGFPExpressionMutationPromoter1.3αCaMKIIAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 SP2
Plasmid#67109PurposeStandard for cell-free expression benchmarking.DepositorInsertSP2 (SP2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only