We narrowed to 2,904 results for: human cdnas

1. pCDNA3.1(+)-CMV-bArrestin2-TEV

   Plasmid

   #107245

   Purpose

   Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ARRB2
   

   Use

   Tags

   TEV(NIA), tobacco etch virus NIA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-FLAG-SBP-STAG2 (WT)

   Plasmid

   #73963

   Purpose

   STAG2 cDNA (CCDS43990) wild type

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3 Lats1 (Nigg HS189)

   Plasmid

   #41156

   Depositor

   Erich Nigg

   Article

   Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

   Insert

   Lats1 (LATS1 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.3−3xFLAG−AGO2(D669A)

   Plasmid

   #220461

   Purpose

   Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Aspartate 669 to alanine

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJA219= pcDNA3.1V5-completeTRABV-CMVp

   Plasmid

   #116878

   Purpose

   mammalian expression vector containing TCR sequences

   Depositor

   Marcos Alcocer

   Article

   Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

   Insert

   complete TRAV10-TRBV25-CMVp rabbitpolyA (TRAV10, TRBV25-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.3−3xFLAG−AGO2(S387A)

   Plasmid

   #220462

   Purpose

   Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Serine 387 to alanine

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.3−3xFLAG−AGO2(S387D)

   Plasmid

   #220463

   Purpose

   Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Serine 387 to aspartate

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-dCas9-p300 Core (D1399Y)

   Plasmid

   #61358

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-dCas9-p300 Core (Y1467F)

   Plasmid

   #61362

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid

    #61361

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-TopBP1-mCer

    Plasmid

    #211414

    Purpose

    mammalian expression of human TopBP1 C-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    TopBP1 (TOPBP1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-TopBP1-mVen

    Plasmid

    #211415

    Purpose

    mammalian expression of human TopBP1 C-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    TopBP1 (TOPBP1 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-RecQL4-mVen

    Plasmid

    #211403

    Purpose

    mammalian expression of human RecQL4 C-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    RecQL4 (RECQL4 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pY120 (pcDNA3.1-huFbCpf1)

    Plasmid

    #92296

    Purpose

    Expression of humanized FbCpf1

    Depositor

    Feng Zhang

    Insert

    huFbCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY126 (pcDNA3.1-huBoCpf1)

    Plasmid

    #92302

    Purpose

    Expression of humanized BoCpf1

    Depositor

    Feng Zhang

    Insert

    huBoCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY123 (pcDNA3.1-huCMaCpf1)

    Plasmid

    #92299

    Purpose

    Expression of humanized CMaCpf1

    Depositor

    Feng Zhang

    Insert

    huCMaCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pY114 (pcDNA3.1-huPgCpf1)

    Plasmid

    #92273

    Purpose

    Expression of humanized PgCpf1

    Depositor

    Feng Zhang

    Insert

    huPgCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pY122 (pcDNA3.1-huCPbCpf1)

    Plasmid

    #92298

    Purpose

    Expression of humanized CPbCpf1

    Depositor

    Feng Zhang

    Insert

    huCPbCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-RecQL4-mCer

    Plasmid

    #211402

    Purpose

    mammalian expression of human RecQL4 C-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    RecQL4 (RECQL4 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-ELMOD3-HA

    Plasmid

    #201902

    Purpose

    Used for expression of human protein ELMOD3 in mammalian cells

    Depositor

    Richard Kahn

    Insert

    ELMOD3 (ELMOD3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge