We narrowed to 703 results for: pcdna3 1 plasmid

1. Kv1.1 K+ channel scFv [K20/78]

   Plasmid

   #182065

   Purpose

   Mammalian Expression of Kv1.1 K+ channel scFv. Derived from hybridoma K20/78.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Kv1.1 K+ channel (Rattus norvegicus) (Kcna1 Mouse)
   

   Use

   Tags

   Sortase, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Arl13b scFv [N295B/66]

   Plasmid

   #182086

   Purpose

   Mammalian Expression of Arl13b scFv. Derived from hybridoma N295B/66.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Arl13b (Mus musculus) (Arl13b Mouse)
   

   Use

   Tags

   HA, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RGS14 scFv [N133/21]

   Plasmid

   #182081

   Purpose

   Mammalian Expression of RGS14 scFv. Derived from hybridoma N133/21.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting RGS14 (Rattus norvegicus) (Rgs14 Mouse)
   

   Use

   Tags

   Sortase, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pY026

   Plasmid

   #84741

   Purpose

   Expresses huAsCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huAsCpf1
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pY027

   Plasmid

   #84742

   Purpose

   Expresses huLbCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huLbCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pY094

   Plasmid

   #84743

   Purpose

   Expresses huAsCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   GFP
   

   Use

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pY30

   Plasmid

   #84745

   Purpose

   Expresses huAsCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Use

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pY095

   Plasmid

   #84744

   Purpose

   Expresses huLbCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   GFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BNLS

    Plasmid

    #50836

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP B
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hHSF1 K298R

    Plasmid

    #118362

    Purpose

    This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K298R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cre Stoplight 2.4

    Plasmid

    #37402

    Purpose

    Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

    Depositor

    Thomas Hughes

    Article

    Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

    Insert

    Cre Stoplight 2.4
    

    Use

    Tags

    ZsGreen1 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hHSF1 S303/7A

    Plasmid

    #118365

    Purpose

    This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A and S307A

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hHSF1 S303A

    Plasmid

    #118363

    Purpose

    This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Kv1.2 K+ channel scFv [K14/16]

    Plasmid

    #182064

    Purpose

    Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GABA(A)R, gamma2 scFv [N452/81]

    Plasmid

    #182088

    Purpose

    Mammalian Expression of GABA(A)R, gamma2 scFv. Derived from hybridoma N452/81.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting GABA(A)R, gamma2 (Homo sapiens) (GABRG2 Mouse)
    

    Use

    Tags

    Sortase, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Mortalin/GRP75 scFv [N52A/42]

    Plasmid

    #182091

    Purpose

    Mammalian Expression of Mortalin/GRP75 scFv. Derived from hybridoma N52A/42.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Mortalin/GRP75 (Mus musculus) (Hspa9 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-K-GECO1

    Plasmid

    #105865

    Purpose

    Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

    Depositor

    Robert Campbell

    Article

    Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

    Insert

    K-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RANLS-DEVD-BNES

    Plasmid

    #50840

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RANLS-IETD-BNES

    Plasmid

    #60972

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only