We narrowed to 59,415 results for: sap

1. Human Coxsackievirus A16 strain G10 inactive 2A protease

   Plasmid

   #228665

   Purpose

   Bacterial expression of the A71 2A protease, innactive and can be used as a control in assays

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Enterovirus A71 2A protease
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Avitag insertion and C2A mutations / Active site …

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. Human Coxsackievirus A16 strain G10 2A protease

   Plasmid

   #228632

   Purpose

   Bacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Enterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pARID4B-donor

   Plasmid

   #132481

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ARID4B (ARID4B Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   none

   Promoter

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIHEU-MCS

   Plasmid

   #58375

   Purpose

   UAS vector to express gene of interest in Drosophila

   Depositor

   Chun Han

   Article

   Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-OR2J2

   Plasmid

   #22321

   Depositor

   Hiroaki Matsunami

   Article

   Saito et al Sci Signal. 2009 . 2(60):ra9.

   Insert

   OR2J2 (OR2J2 Human)
   

   Use

   Tags

   Rho

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLS268

   Plasmid

   #73726

   Purpose

   SapTrap SL2 C-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   SL2 (gdp-2/gdp-3 intergenic region)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMLS285

   Plasmid

   #73734

   Purpose

   SapTrap egl-13 NLS connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   egl-13 NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. WESSV strain SAH177 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228659

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Wesselsbron virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Wesselsbron virus portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. SPOV strain SM-6 V-1 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228656

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Spondweni virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Spondweni virus portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. DENV-4 strain H241 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228652

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 4 for biochemical assays

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue 4 portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. DENV-2 strain Thailand/16681/84 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228650

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 2 for biochemical assays

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue 2 portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain

    Plasmid

    #228643

    Purpose

    Bacterial expression of the SARS-CoV-2 nsp3 macrodomain for crystallization of SARS-CoV-2 nsp3 macrodomain in P 4 3

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    SARS-CoV-2 NSP3 macrodomain (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag

    Plasmid

    #228635

    Purpose

    Bacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interaction

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Enterovirus A71 3C protease isoform A
    

    Use

    Tags

    His-Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag

    Plasmid

    #228634

    Purpose

    Bacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interaction

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Enterovirus Coxsackievirus A71 2A protease
    

    Use

    Tags

    His-Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pARID4B.1.0-gDNA

    Plasmid

    #132436

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ARID4B (ARID4B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124283

    Purpose

    sgRNA against human Thy-1

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

17. Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124285

    Purpose

    sgRNA against human Thy-1

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

18. Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124284

    Purpose

    sgRNA against human Thy-1

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

19. 5JJW (USP15_SART3)

    Plasmid

    #101880

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP15_SART3 (USP15, SART3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. APEX2-NLS

    Plasmid

    #124617

    Purpose

    proximity biotinylation

    Depositor

    Alice Ting

    Article

    Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

    Insert

    APEX2-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge