We narrowed to 1,221 results for: sumo

1. GFP-(SIM)3

   Plasmid

   #126955

   Purpose

   Bacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. (SIM)5

   Plasmid

   #126947

   Purpose

   Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. (SIM)10

   Plasmid

   #126946

   Purpose

   Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTU422

   Plasmid

   #178982

   Purpose

   13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Inserts

   gRAMP
   CRISPR array
   

   Use

   Tags

   Strep-Tag II SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. hHSF4b WT

   Plasmid

   #118356

   Purpose

   This plasmid expresses human HSF4b WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hHSF4b (HSF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-(SIM)1

   Plasmid

   #126954

   Purpose

   Bacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RFP-(SIM)1

   Plasmid

   #127094

   Purpose

   Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   mCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 SPASTIN , residues 108-200

   Plasmid

   #180628

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 SPASTIN , residues 112-196

   Plasmid

   #180627

   Purpose

   Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 112-196

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-TEV-UBC-9

    Plasmid

    #87055

    Purpose

    Expresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    UBC-9 (ubc-9 Nematode)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-HsSENP3(307-574)

    Plasmid

    #71481

    Purpose

    Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-306 (catalytic domain only)

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-PML RB1

    Plasmid

    #185636

    Purpose

    expression of GST-tagged PML fragment 49-168 (RING + B-box1) in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

    Insert

    promyelocytic leukemia protein (PML Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT/T0-3XFLAG-NESm-NFATC2IP

    Plasmid

    #221445

    Purpose

    Mammalian expression vector of triple Flag tagged NFATC2IP ORF with mutant nuclear export signal (NESm) at N-terminus

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Tags

    3XFlag and Mutant Nuclear Export Signal (mNES)

    Expression

    Mammalian

    Mutation

    mutant NES (NESm: NLVDLQKKLEEAKADEQQ (PMID: 10739…

    Promoter

    CMV

    Available since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pcDNA5-FRT/T0-eGFP-NES-NFATC2IP

    Plasmid

    #221446

    Purpose

    Mammalian expression vector of eGFP tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Tags

    Nuclear Export Signal (NES) and eGFP

    Expression

    Mammalian

    Mutation

    NES (NLVDLQKKLEELELDEQQ) inserted, 1st Methionine…

    Promoter

    CMV

    Available since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA5-FRT/T0-3xFLAG-NFATC2IP D394R

    Plasmid

    #221211

    Purpose

    Mammalian expression vector of triple Flag tagged NFATC2IP ORF with D394R point mutation. Charge-reverting mutation for UBC9 R13D mutant for interaction

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    D394R (charge-reverting mutation for interaction …

    Promoter

    CMV

    Available since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pcDNA5-FRT/T0-eGFP-NFATC2IP D394R

    Plasmid

    #221436

    Purpose

    Mammalian expression vector of eGFP tagged NFATC2IP ORF with D394R point mutation. Charge-reverting mutation for UBC9 R13D mutant for interaction

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    D394R (charge-reverting mutation for interaction …

    Promoter

    CMV

    Available since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA5-FRT/T0-3XFLAG-NES-NFATC2IP

    Plasmid

    #221444

    Purpose

    Mammalian expression vector of triple Flag tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Tags

    3XFlag and Nuclear Export Signal (NES)

    Expression

    Mammalian

    Mutation

    NES (NLVDLQKKLEELELDEQQ)inserted, 1st Methionine …

    Promoter

    CMV

    Available since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCA528 USP54, residues 731-878

    Plasmid

    #180637

    Purpose

    Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    USP54 (USP54 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDB179

    Plasmid

    #91960

    Purpose

    empty expression vector for 10His-SUMO tag protein purification

    Depositor

    Christopher Bahl

    Type

    Empty backbone

    Use

    Tags

    10-His and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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