We narrowed to 12,152 results for: 112

1. pJL1-CRM197-4xDQNAT

   Plasmid

   #128395

   Purpose

   In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Diphtheria toxin
   

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   Inactivating G52E mutation

   Promoter

   T7

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFCcGi

   Plasmid

   #59324

   Purpose

   Inducible expression of GFP and constitutive expression of mCherry

   Depositor

   Sophie Helaine, David Holden

   Article

   Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

   Inserts

   mCherry
   EGFP
   

   Expression

   Bacterial

   Promoter

   Pbad and rpsM

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0040-LwaCas13a crRNA backbone

   Plasmid

   #103851

   Purpose

   Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-LwaCas13aDR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. C1-MPAct-mCherry

   Plasmid

   #155222

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMF-TetTight

   Plasmid

   #193288

   Purpose

   Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

   Depositor

   Rudolf Jaenisch

   Article

   Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

   Insert

   rtTA-P2A-tTS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF‐1α

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDH

   Plasmid

   #133129

   Purpose

   A recombinant plasmid expressing mdh (malate dehydrogenase) gene

   Depositor

   Gregg Beckham

   Article

   Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   malate dehydrogenase gene
   

   Expression

   Bacterial

   Promoter

   malate dehydrogenase promoter

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMN-333

   Plasmid

   #195467

   Purpose

   Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   inverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDN-Cherry-sgRNA

   Plasmid

   #221387

   Purpose

   episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   none, cloning vector for introduction of targeting sequences
   

   Use

   CRISPR

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRISPR_HLA_class_I_1

   Plasmid

   #164986

   Purpose

   Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against HLA class I
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJMP8835

    Plasmid

    #220902

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMZ-180

    Plasmid

    #195428

    Purpose

    Transient, constitutive expression of synZiFTR: ZF5-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF5-7 (ZF5) - p65 - RbGpA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-ZDHHC11

    Plasmid

    #231659

    Purpose

    Expresses human ZDHHC11 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC11 (ZDHHC11 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMN-013

    Plasmid

    #195417

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF5

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF5 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMN-332

    Plasmid

    #195466

    Purpose

    Lentiviral Integratable Inducible synZiFTR ZF1 + Reporter

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF1 BS - ybTATA - mCherry] - pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAsp-AMA-gpdA-ptr

    Plasmid

    #191016

    Purpose

    CRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi)

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

    Type

    Empty backbone

    Use

    Unspecified; Aspergillus

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-IRES-GFP-mouse-Caspase-11

    Plasmid

    #183358

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-11 (Casp4 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Promoter

    MESV

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-YU2gp160

    Plasmid

    #100927

    Purpose

    Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (YU2) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-IRES-GFP-human-Caspase-4

    Plasmid

    #183356

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Promoter

    MESV

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTrex-n-eGFP

    Plasmid

    #62544

    Purpose

    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome

    Depositor

    Rick Tarleton

    Article

    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

    Insert

    eGFP
    

    Use

    (1)trypanosoma cruzi expression of egfp (2) genom…

    Tags

    None

    Mutation

    None

    Promoter

    Ribo-HX1

    Available Since

    April 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCiPS-NIFV

    Plasmid

    #110807

    Purpose

    Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells

    Depositor

    David Russell

    Article

    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

    Insert

    Pol
    

    Expression

    Mammalian

    Mutation

    2 mutations in the DDE catalyic core motif of the…

    Promoter

    CMV

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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