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pSEVA19_LacI_pLac-sgRNA-1

Plasmid

#231408

Purpose

sgRNA-1 expression from an IPTG-inducible promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
LacI

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_AraC_pBAD-sgRNA-1

Plasmid

#231407

Purpose

sgRNA-1 expression from an arabinose-inducible promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
AraC

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Zdk3-VP64

Plasmid

#231571

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Zdk3-VP64

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_Zdk3-VPR

Plasmid

#231573

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Zdk3-VPR, CMV driven

Expression

Mammalian

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S1S2 [backbone]

Plasmid

#229194

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl2-[lenti-backbone]

Plasmid

#229208

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I-E1

Plasmid

#229161

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

E1

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I-F1

Plasmid

#229162

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

F1

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I [backbone]

Plasmid

#229153

Purpose

Insulator backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-P [backbone]

Plasmid

#229154

Purpose

Promoter backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-5 [backbone]

Plasmid

#229155

Purpose

5 prime backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G [backbone]

Plasmid

#229156

Purpose

Gene backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-3 [backbone]

Plasmid

#229157

Purpose

3 prime backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-T [backbone]

Plasmid

#229158

Purpose

Terminator backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-1_M13ps

Plasmid

#231409

Purpose

M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-1

Use

Synthetic Biology

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGH907

Plasmid

#230837

Purpose

Ef1a-MS2-AIDDead-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

MS2-AIDDead

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH518

Plasmid

#230836

Purpose

Ef1a-MS2-AID*-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

MS2-AID*

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH896

Plasmid

#230840

Purpose

Ef1a-DivA-BE-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH941

Plasmid

#230846

Purpose

Ef1a-DivA-BE-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH897

Plasmid

#230841

Purpose

Ef1a-nDivA-BE-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

nDivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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