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pEMS2181

Plasmid

#158421

Purpose

AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple32 (CLDN5 Human)

Use

AAV

Promoter

Ple32

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB

Plasmid

#207859

Purpose

AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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PA-Dre

Plasmid

#169706

Purpose

Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

Dre246-nMag-T2A-pMag-Dre247

Use

AAV

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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hSyn-GtACR2-ChRmine-tandem-eYFP

Plasmid

#192580

Purpose

AAV-vector for bidirectional optogenetic manipulation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

somBiPOLES

Use

AAV

Tags

EYFP- soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

human synapsin

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2113

Plasmid

#49138

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-emGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

EF1a-short; U6

Available Since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG

Plasmid

#206956

Purpose

AAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

mScarlet-I, MmMT3, QtEnc

Use

AAV

Tags

FLAG

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2230

Plasmid

#158420

Purpose

AAV plasmid with Ple253 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple253 (PITX3 )

Use

AAV

Promoter

Ple253

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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hSyn1-AsCpf1(RR)

Plasmid

#109325

Purpose

AAV vector expressing AsCpf1 (RR variant)

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

hAsCpf1(RR)

Use

AAV

Tags

HA and NLS

Mutation

S542R/K607R

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1a-CRY2PHR-SID4X

Plasmid

#63663

Purpose

AAV expression of CRYS2PHR-SID4X

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Insert

SID4X-CYR2PHR

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(TBK1)

Plasmid

#109322

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (TBK1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS2382

Plasmid

#225638

Purpose

AAV plasmid with Ple384 (TH MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Galvan et al Sci Rep. 2026 Feb 16. doi: 10.1038/s4159

Insert

Ple384 (TH Human)

Use

AAV

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-mGrm3-WPRE

Plasmid

#224236

Purpose

AAV expression of mGrm3 from GfaABC1D promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mGrm3 (Grm3 Mouse)

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-flag-mAdrb1-mNeonGreen-WPRE

Plasmid

#223672

Purpose

AAV expression of flag-mAdrb1-mNeonGreen from GfaABC1D promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mAdrb1-mNeonGreen (Adrb1 Mouse)

Use

AAV

Tags

FLAG and mNeonGreen

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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MKC84_HBB(HBA1reg-HBA1)

Plasmid

#232410

Purpose

AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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MKC83_HBB(HBBreg-HBA1)

Plasmid

#232409

Purpose

AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA

Plasmid

#194247

Purpose

Expresses 3 miRNAs targeting human alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(human) (SNCA Human)

Use

AAV and RNAi

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

Plasmid

#194248

Purpose

Expresses 3 miRNAs targeting mouse alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(mouse) (Snca Mouse)

Use

AAV and RNAi

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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dTomato_LD 0_TEV

Plasmid

#58872

Purpose

MESA protease chain with dTomato ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with dTomato ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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AV0-EF1-N-cG

Plasmid

#192888

Purpose

Expression of Nanoluc-P2A-copGFP

Depositor

Kazuhiro Oka

Article

Kulkarni et al Bio Protoc. 2024 Feb 5;14(3):e4931. doi:

Insert

Nanoluc-P2A-copGFP

Use

AAV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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