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pSCT-GAL93-PSPC1

Plasmid

#46321

Depositor

Steven Brown

Publication

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PSPC1 (PSPC1 Human)

Expression

Mammalian

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-214

Plasmid

#26313

Depositor

David Bartel

Publication

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-214 (MIR214 Human)

Expression

Mammalian

Available since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CSNK1G3

Plasmid

#23400

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK1G3 (CSNK1G3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIK3C3

Plasmid

#23457

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3C3 (PIK3C3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PI4K2B

Plasmid

#23507

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PI4K2B (PI4K2B Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
STAGR_gRNAScaffold_mU6

Plasmid

#102844

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Publication

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_mU6

Use

Pcr template for stagr inserts

Promoter

mU6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFH6

Plasmid

#86555

Purpose

Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

Depositor

Andreas Weber

Publication

Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

Insert

U6-26p::sgRNA scaffold

Use

CRISPR; Subcloning

Promoter

Arabidopsis U6-26 promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_WT

Plasmid

#82133

Purpose

Gateway Donor vector containing IDH1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pME-memGFP-P2A

Plasmid

#80809

Purpose

Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pME-memGFP-P2A

Expression

Mammalian

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAC-EPSILON

Plasmid

#53276

Purpose

Contains ctrE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and the lcyE gene of Lactuca sativa and thereby produces epsilon-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

lcyE

Use

Low copy number bacterial cloning vector

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-TAOK3

Plasmid

#23861

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TAOK3 (TAOK3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ADRBK2

Plasmid

#23793

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADRBK2 (AA1-387) (GRK3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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PB-tetO-NB

Plasmid

#197092

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

Depositor

Brian Wainger

Publication

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)

Expression

Mammalian

Promoter

TRE3G

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-HLA-E SCT-T2A-GFP

Plasmid

#205461

Purpose

lentiviral plasmid for expression of HLA-E SCT

Depositor

Deepta Bhattacharya

Publication

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HLA-E SCT (HLA-E Human)

Expression

Mammalian

Promoter

UBC

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-LC3

Plasmid

#110947

Purpose

Expresses the autophagosome marker LC3 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Publication

Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

mTurquoise2

Expression

Mammalian

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Klf2-t2A-mCherry

Plasmid

#127539

Purpose

Expresses mouse Klf2 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Publication

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Klf2 (Klf2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-p62(ΔUBD)

Plasmid

#119687

Purpose

For mammalian expression of p62 without its UBD

Depositor

Michael Lazarou

Publication

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

UBD deletion (1-385)

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _3xHA-Ago2

Plasmid

#73538

Purpose

Inducible lentiviral expression of Ago2

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K13-15-118-119R

Plasmid

#63568

Purpose

Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

Depositor

Titia Sixma

Publication

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 13, 15, 118, and 119 are now Arginine

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
PuroR-P2A-DD-TCOF1-donor

Plasmid

#60747

Purpose

Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

Depositor

Benhur Lee

Publication

Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

Insert

TCOF1 (TCOF1 Human)

Use

Targeting donor

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only