We narrowed to 19,144 results for: tec
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TAL3164
Plasmid#41338DepositorInsertZebrafishCommunity-slc26a6-Left (LOC100005108 Zebrafish)
Uset7Tags3X Flag and WT FOKIExpressionMammalianPromoterCMVAvailable SinceNov. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pMT468
Plasmid#139394PurposeNanoluc placed on Output 2 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs, AmpR, ErmRDepositorInsertNanoluc placed on Output 2 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs
UseSynthetic BiologyPromoteraTC and BA inducible promotersAvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE
Plasmid#138150PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralMutationDR1 A>TAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDB_054
Plasmid#216096PurposeAll-in-one, knockout, delivers Cas12a & gRNADepositorInsertCas12a [EnAs]
UseCRISPR and Lentiviral; Assembled vectorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)::RAM-d2tTA
Plasmid#189627PurposeExpresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.DepositorInsertshM3D(Gq)
d2tTA
UseAAVTagsHAExpressionMammalianPromoterhSyn and pRAMAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1765 (pSPIN-R, R*MmeI)
Plasmid#160734PurposeSingle-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-miniXon_Luciferase
Plasmid#174660PurposePlasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.DepositorInsertminiXon cassette
UseLuciferaseExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1
Plasmid#187120Purposemammalian cell expression of FLAG-HUWE1DepositorAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552
Plasmid#60958PurposepAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.DepositorInsertsU6_(SpaI)_sgRNA
Syn_EGFP-KASH
UseAAV, CRISPR, and Mouse TargetingExpressionMammalianPromoterSynapsin and U6Available SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-Xon_Luciferase
Plasmid#174659PurposePlasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.DepositorInsertXon cassette
UseLuciferaseExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_FLAG-HUWE1
Plasmid#187121Purposemammalian cell expression of FLAG-HUWE1DepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMV_AA050
Plasmid#216107PurposegDNA expression for Cas9 with ChenF+E trRNA (guide only)DepositorInsertgDNA expression for Cas9 with ChenF+E trRNA
UseCRISPR and Lentiviral; Assembled vectorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)
Plasmid#138555PurposeExpresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSDDepositorInserteSpCas9(1.1)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationK848A, K1003A, R1060APromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only
