We narrowed to 7,572 results for: Lif

1. pDest27-mBIN1+13

   Plasmid

   #61087

   Purpose

   Express N-terminal GST tagged-mouse BIN1+13 in mammalian cells

   Depositor

   Robin Shaw

   Article

   Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

   Insert

   mouse BIN1 with exon 13, excluding exon 7, 11, 14-17
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDest27-mBIN1

   Plasmid

   #61086

   Purpose

   Express N-terminal GST tagged-mouse BIN1 in mammalian cells

   Depositor

   Robin Shaw

   Article

   Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

   Insert

   smallest constitutive BIN1excluding exon 7, 11, 13-17
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

3. L-RARS-5

   Plasmid

   #166535

   Purpose

   scFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. L-KARS-5

   Plasmid

   #166530

   Purpose

   scFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-length

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. O-DARS-1

   Plasmid

   #166538

   Purpose

   scFv of a human scaffold targeting Aspartyl-tRNA synthetase. Antigen coverage aa 1-501 of 501, full-length

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. O-LARS-15

   Plasmid

   #166542

   Purpose

   scFv of a human scaffold targeting Leucyl-tRNA synthetase. Antigen coverage aa 260-509 of 1176

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KRAS2B

   Plasmid

   #55655

   Purpose

   expression of mouse KRAS2B

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   KRAS2B (Kras Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA_DiCre_60.F2

   Plasmid

   #60208

   Purpose

   Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

   Depositor

   Jean-Paul Herman

   Article

   Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

   Insert

   Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)
   

   Use

   Tags

   FRAP and Nuclear localisation signal

   Expression

   Mammalian

   Mutation

   amino acids 2021-2112 of FRAP with a T2098L mutat…

   Promoter

   CMV

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGG-TurboID-GFP-PTS1

   Plasmid

   #209406

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID-PTS1
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-6P-2_dCrk

    Plasmid

    #131146

    Purpose

    Expresses GST::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR P4-P1R-mKate2

    Plasmid

    #48344

    Purpose

    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    mKate2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    It contains a Kozak sequence but no stop codon.

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-SF3B1-NTerm

    Plasmid

    #97425

    Purpose

    Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

    Depositor

    Alex Minella

    Article

    Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

    Insert

    SF3B1-N-term (AA1-500)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Silent mutation at nt876, aa289 (AGG to AGA)

    Promoter

    tac

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS ColIII alpha1

    Plasmid

    #19187

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Procollagen type III alpha 1 (COL3A1 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pBS GDF10

    Plasmid

    #19186

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Growth differentiation factor 10 precursor (GDF10 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pDest27-mBIN1+17

    Plasmid

    #61096

    Purpose

    Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

    Depositor

    Robin Shaw

    Article

    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

    Insert

    mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

16. L-YARS2-9

    Plasmid

    #166533

    Purpose

    scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RCAS(A) dRhoA V14 (CT#846)

    Plasmid

    #13949

    Depositor

    Cliff Tabin

    Article

    Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

    Insert

    RhoA (Rho1 Fly)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    G14V mutation

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pGS1022

    Plasmid

    #59576

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    

    Use

    E. coli cloning vector

    Tags

    Twin-Strep tag

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. O-MARS-11

    Plasmid

    #166541

    Purpose

    scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCru5-/GCCACC-mEGFP-IRES-mCherry

    Plasmid

    #49226

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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