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pMTU-DO-HPH-C4

Plasmid

#160343

Purpose

High-copy expression vector for K. marxianus, hygromycin selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-URA-C4

Plasmid

#160339

Purpose

High-copy expression vector for K. marxianus, uracil selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR'-NRSF-CITE-GFP

Plasmid

#21310

Depositor

Jay Nadeau

Article

Nadeau et al J Neurophysiol. 2002 Jul . 88(1):409-21.

Insert

neuron restrictive silencer factor (Rest Mouse)

Use

Lentiviral

Tags

GFP (bicistronic with CITE/IRES)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Oct. 20, 2009

Availability

Academic Institutions and Nonprofits only

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p285 TIR1_HA_mH11

Plasmid

#164855

Purpose

constitutive TIR1 expression vector for mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-HIS-C4

Plasmid

#160340

Purpose

High-copy expression vector for K. marxianus, histidine selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-LEU-C4

Plasmid

#160341

Purpose

High-copy expression vector for K. marxianus, leucine selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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p274 EF1a_EGFP_hH11

Plasmid

#164851

Purpose

EGFP-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

EGFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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p281 TIR1_HA_flox STOP_mH11

Plasmid

#164856

Purpose

Cre-inducible TIR1 expression vector for mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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p273 EF1a_mCherry2_hH11

Plasmid

#164852

Purpose

mCherry2-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

mCherry2

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 - mSRSF6

Plasmid

#120420

Purpose

overexpression of mouse Srsf6

Depositor

Gillian Bates, Andreas Neueder

Article

Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

Insert

SRSF6 (Srsf6 Mouse)

Expression

Mammalian

Promoter

CMV immediate/early

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pBD-0016

Plasmid

#12345

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP R313K (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

R313K, I206V*(see below)

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
I-2/pCS2MT

Plasmid

#16317

Depositor

Peter Klein

Article

Zhang et al J Biol Chem. 2003 Aug 29. 278(35):33067-

Insert

Inhibitor-2 (Ppp1r2 Mouse)

Use

Vertebrate expression

Tags

5x myc

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pQE9-His-p97(QQ)

Plasmid

#14667

Depositor

Graham Warren

Article

Ye et al Nature. 2001 Dec 6. 414(6864):652-6.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

E305/578Q

Available Since

April 13, 2007

Availability

Academic Institutions and Nonprofits only
pISH-Trpc2-probe2

Plasmid

#105474

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Cell Rep. 2014 Jul 24;8(2):583-95. doi:

Insert

Trpc2 (Trpc2 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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p817-mgammaFcry-mCherry

Plasmid

#23154

Depositor

Zbynek Kozmik

Article

Vopalensky et al Biotechniques. 2010 Mar . 48(3):235-6.

Insert

mouse gammaF crystallin promoter:mCherry (Crygf Mouse)

Use

Multicloning site flanked by i-scei sites

Tags

mCherry

Mutation

mouse gammaF crystallin promoter (-225 - +73) pre…

Available Since

April 13, 2010

Availability

Academic Institutions and Nonprofits only

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p286 TIR1_HA_hH11

Plasmid

#164853

Purpose

constitutive TIR1 expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGDP2 MCR-1

Plasmid

#118404

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

MCR-1

Expression

Bacterial

Promoter

pLac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGDP1 fosA

Plasmid

#112889

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

fosA

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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VPS13D^mScarlet

Plasmid

#174110

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Tags

mScarlet

Expression

Mammalian

Mutation

With an internal mScarlet tag in aminoacid positi…

Promoter

CMV

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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VPS13D^EGFP

Plasmid

#174109

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Tags

EGFP

Expression

Mammalian

Mutation

With an internal EGFP tag in aminoacid position 1…

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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