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pHAGE-EZH2-N322S

Plasmid

#116375

Purpose

Lentiviral expression of EZH2 N322S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EZH2 (EZH2 Human)

Use

Lentiviral

Mutation

N322S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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B52 + FANCM sgSTOP

Plasmid

#100710

Purpose

B52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDX3X-S412F

Plasmid

#116227

Purpose

Lentiviral expression of DDX3X S412F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Mutation

S412F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-P539R

Plasmid

#116525

Purpose

Lentiviral expression of PIK3CA P539R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

P539R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDX3X-E285K

Plasmid

#116226

Purpose

Lentiviral expression of DDX3X E285K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Mutation

E285K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-L747_P753delinsS

Plasmid

#116268

Purpose

Lentiviral expression of EGFR L747_P753delinsS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

L747_P753delinsS

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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BKbeta2 K+ channel scFv [N53/32]

Plasmid

#190561

Purpose

Mammalian Expression of BKbeta2 K+ channel scFV. Derived from hybridoma N53/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

BKbeta2 K+ channel (Mus musculus) recombinant scFV (Kcnmb2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-NSD1 [N312/10]

Plasmid

#190311

Purpose

Mammalian Expression Plasmid of anti-NSD1 (Human). Derived from hybridoma N312/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NSD1 (Homo sapiens) recombinant Mouse monoclonal antibody (NSD1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-WRN [N428/12R]

Plasmid

#206575

Purpose

Mammalian Expression Plasmid of anti-WRN (Human). Derived from hybridoma N428/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-WRN (Homo sapiens) recombinant Mouse monoclonal antibody (WRN Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-T241P

Plasmid

#116197

Purpose

Lentiviral expression of BRAF T241P

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

T241P

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-T599I

Plasmid

#116201

Purpose

Lentiviral expression of BRAF T599I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

T599I

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Calbindin scFv [L109/57]

Plasmid

#182071

Purpose

Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600_K601delinsE

Plasmid

#116203

Purpose

Lentiviral expression of BRAF V600_K601delinsE

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600_K601delinsE

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pQhsCDtRNA+

Plasmid

#86014

Purpose

Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene

Depositor

Hong Li

Article

Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

Inserts

U14-tRNA chimera (SNORD14A Human)
15.5K (SNU13 Human)
fibrillarin (FBL Human)
NOP56 (NOP56 Human)
NOP58 (NOP58 Human)

Use

Co-expression; purification

Tags

6x-His

Expression

Bacterial

Mutation

amino acids 1-401, amino acids 1-411, and amino a…

Promoter

LPP and Ptac

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-AXL-C311F

Plasmid

#116116

Purpose

Lentiviral expression of AXL C311F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AXL (AXL Human)

Use

Lentiviral

Mutation

C311F

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT1-R121W

Plasmid

#116104

Purpose

Lentiviral expression of AKT1 R121W

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Mutation

R121W

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E110delE

Plasmid

#116469

Purpose

Lentiviral expression of PIK3CA E110delE

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

E110delE

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_9

Plasmid

#60258

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

NKX6-1 enhancer (NKX6-1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-N345K

Plasmid

#116517

Purpose

Lentiviral expression of PIK3CA N345K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

N345K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600M

Plasmid

#116208

Purpose

Lentiviral expression of BRAF V600M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600M

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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