We narrowed to 8,324 results for: gnal

1. pN1-mCherry-NKD2

   Plasmid

   #176641

   Purpose

   Expresses NKD2-mCherry fusion protein in mammalian cells

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
   

   Use

   Tags

   mCherry tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMpGWB216

   Plasmid

   #68607

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Nuclear localization signal-tagged tdTomato (tdTo…

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CD4_LD 0_CS G_tTA-BFP

   Plasmid

   #58869

   Purpose

   MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ERKsubDD-Venus-NES

   Plasmid

   #84630

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   ERKsubDD-Venus-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTrc99S-ssDsbA-PD-4xDQNAT

   Plasmid

   #128399

   Purpose

   Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   H. influenzae protein D
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTrc99S-ssDsbA-PorA-4xDQNAT

   Plasmid

   #128400

   Purpose

   Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   N. meningitidis PorA
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJBL3664

   Plasmid

   #162240

   Purpose

   Transcribes E. coli SRP RNA sequence for SHAPE-Seq

   Depositor

   Julius Lucks

   Article

   Yu et al Mol Cell. 2021 Feb 18;81(4):870-883.e10.

   Inserts

   E. coli SRP RNA
   HDV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDB368

   Plasmid

   #110276

   Purpose

   gHH_44 with a N-terminal 10-His DsbC TEV-peptide fusion

   Depositor

   Christopher Bahl

   Article

   Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

   Insert

   gHH_44
   

   Use

   Tags

   10-His, Tev recognition peptide, and mature DsbC …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-RLuc8C-FHA1-NES

   Plasmid

   #138210

   Purpose

   Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   RLuc8C-FHA1-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mCherry-PKAsub-RLuc8N-NES

    Plasmid

    #138211

    Purpose

    Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    mCherry-PKAsub-RLuc8N-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PA-mCherry-miniSOG-Peroxisomes-2

    Plasmid

    #54847

    Purpose

    Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    PA-mCherry-miniSOG and Peroximal Targeting Signal…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

12. mCherry_LD 0_CS M_tTA-BFP

    Plasmid

    #58874

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT1100

    Plasmid

    #8618

    Depositor

    Robert Hartley

    Article

    Hartley et al Protein Peptide Letters 1996 3:225-231

    Insert

    ribonuclease Sa3, barstar
    

    Use

    Tags

    phoA signal sequence to RNase Sa3

    Expression

    Bacterial

    Mutation

    In addition to the substitution of the barnase ge…

    Promoter

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

14. mCherry_LD 0_CS A_tTA-BFP

    Plasmid

    #58873

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCNL-N1_3xNLS

    Plasmid

    #65673

    Purpose

    Expresses NLS-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYNL-N1_3xNLS

    Plasmid

    #65672

    Purpose

    Expresses NLS-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNUT N6His mICA NG

    Plasmid

    #70162

    Purpose

    Expresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydrase

    Depositor

    Anne Mason, Ross MacGillivray

    Article

    Wang et al Biochem J. 2007 Aug 15;406(1):85-95.

    Insert

    mouse inhibitor of carbonic anhydrase
    

    Use

    Tags

    N-terminal signal peptide, 4 aa link, 6 His, Fact…

    Expression

    Mammalian

    Mutation

    Asn470Asp and Asn645Asp

    Promoter

    SV40

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pONL-N1_3xNLS

    Plasmid

    #65674

    Purpose

    Expresses NLS-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. SP_C3

    Plasmid

    #228159

    Purpose

    Plasmid contains the secretion signal peptide sequence of the yeast (strain ATCC 204508 / S288c) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_Q03178_PIR1_YEAST:0-18
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210734

    Purpose

    Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Frodo sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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