Addgene

Showing: 341 - 350 of 21252 results

Results per page:

Sort results by:

pKLV2-U6gRNA5(SMARCA4(43))-PGKpuro2ABFP-W

Plasmid

#200464

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43) (SMARCA4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(SMARCA2(46))-PGKpuro2ABFP-W

Plasmid

#200465

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA2(46) (SMARCA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23PI-Sce ESARC D218A

Plasmid

#195608

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Use

Tags

Expression

Bacterial

Mutation

T928C, T929C, A942C

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7PI-Sce ESARC D326A

Plasmid

#195609

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Use

Tags

Expression

Bacterial

Mutation

T3593C, A3601C, T3602G

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-SIRPT1-Sarcophilus harrisii

Plasmid

#75320

Purpose

GST fusion Sarcophilus harrisii Sacsin 90-1369

Depositor

Kalle Gehring

Insert

Sacsin Sarcophilus harrisii 90-1369

Use

Tags

GST-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pColdII-SIRPT1-Sarcophilus harrisii

Plasmid

#75352

Purpose

Nt His-tag Sarcophilus harrisii Sacsin 90-1369

Depositor

Kalle Gehring

Insert

Sacsin Sarcophilus harrisii 90-1369

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)

Plasmid

#79785

Purpose

genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a barcode landing pad

Depositor

Anthony Zador

Insert

MAPP-nL

Use

Synthetic Biology; Sindbis virus genomic plasmid

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFAST-BAC BAF155/SMARCC1-His

Plasmid

#177863

Purpose

Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

Depositor

Yoshihiro Izumiya

Article

Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

Insert

SMARCC1 (SMARCC1 Human)

Use

Tags

His and His tag at C-terminus with GGGGS linker

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF143-wArchon1-KGC-EGFP-ER2

Plasmid

#108429

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon1-KGC-EGFP-ER2

Use

Tags

Expression

Worm

Mutation

Promoter

flp-18

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

Plasmid

#170229

Purpose

Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Q64L

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge