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pKDsgRNA-arsB

Plasmid

#89952

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

arsB gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-Ala1

Plasmid

#65814

Purpose

NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Insulated waaHp from E. coli

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Escherichia coli, Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-PDI

Plasmid

#202486

Purpose

Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Cav1 in situ probe

Plasmid

#45624

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Cav1 in situ probe (Cav1 Mouse)

Use

In situ

Mutation

fragment contains bp# 1472-2124 of AB029929.1

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pEM-Cas9noSsrA

Plasmid

#102285

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Mutation

removed ssrA tag

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Btg1

Plasmid

#45865

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Btg1 in situ probe (Btg1 Mouse)

Use

In situ

Mutation

contains bp# 1004-1638 of BC006834.1

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Chn2 in situ probe

Plasmid

#45625

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Chn2 in situ probe (Chn2 Mouse)

Use

In situ

Mutation

fragment contains bp# 2212-2619 of BC051139.1

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Inhba

Plasmid

#45868

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Inhba in situ probe (Inhba Mouse)

Use

In situ

Mutation

nt 428-933 from Inhba mRNA (NM_008380.1)

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only
pCASCADE-gltA1-gltA2

Plasmid

#71348

Purpose

gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

gltA1-gltA2 guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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