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C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

Plasmid

#162933

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-maltose binding protein fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

Plasmid

#159579

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

Plasmid

#159540

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12C

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

Plasmid

#159698

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X52-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169)

Plasmid

#159560

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)

Plasmid

#159580

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)

Plasmid

#30123

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-MBP-N10-TEV

Expression

Insect

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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Gal1/10 His6-MBP TEV Ura S. cerevisiae expression vector (12URA-C)

Plasmid

#48305

Depositor

Scott Gradia

Type

Empty backbone

Use

Na

Tags

His6 and MBP

Expression

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

Plasmid

#55209

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

10xAsn, 10xHis, MPB, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

Plasmid

#159548

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12D

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

Plasmid

#48311

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

Plasmid

#159541

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12D

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R989-X10-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V

Plasmid

#159542

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12V

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R989-X11-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D

Plasmid

#159543

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G13D

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R791-X12-636: His6-MBP-tev-Hs.NF1 ABC (2-1540)

Plasmid

#159577

Purpose

Baculovirus protein expression of His6-MBP-tev-Hs.NF1 ABC (2-1540)

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-MBP-tev-Hs.NF1 ABC (2-1540) (NF1 Human)

Use

Tags

His6-MBP-tev

Expression

Insect

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R989-X13-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R

Plasmid

#159545

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Q61R

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X31-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Plasmid

#159551

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Q61L

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R989-X12-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L

Plasmid

#159544

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Q61L

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

Plasmid

#159692

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

Depositor

Dominic Esposito

Article

Procter et al Protein Expr Purif. 2018 Nov;151:99-105.

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Insect

Mutation

M188L

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
R989-X30-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

Plasmid

#159550

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G13D

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge