We narrowed to 14,014 results for: TIM

1. pNB785

   Plasmid

   #60164

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   SIC1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNB787

   Plasmid

   #60152

   Purpose

   Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LEU1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNB789

   Plasmid

   #60166

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   RNR1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNB682

   Plasmid

   #60143

   Purpose

   Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRiboI-Sec-APEX2m

   Plasmid

   #176845

   Purpose

   Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-Sec-APEX2m
   

   Expression

   Bacterial

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-opEBNA2-IRES-mCherry

   Plasmid

   #220355

   Purpose

   Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   codon-optimised for expression in mammalian cells

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRibo-Sec-APEX2m

   Plasmid

   #176844

   Purpose

   Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-Sec-APEX2m
   

   Use

   Inducible

   Expression

   Bacterial

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLT 651

   Plasmid

   #59998

   Purpose

   Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

   Depositor

   Lisa Timmons

   Article

   Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

   Inserts

   klp-16 (klp-16 Nematode)
   him-8 (him-8 Nematode)
   

   Expression

   Bacterial

   Mutation

   partial genomic

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLT 653

   Plasmid

   #59996

   Purpose

   Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

   Depositor

   Lisa Timmons

   Article

   Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

   Inserts

   dhc-3 (dhc-3 Nematode)
   klp-16 (klp-16 Nematode)
   

   Expression

   Bacterial

   Mutation

   partial genomic

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLT 652

    Plasmid

    #59997

    Purpose

    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

    Depositor

    Lisa Timmons

    Article

    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

    Inserts

    dhc-3 (dhc-3 Nematode)
    him-8 (him-8 Nematode)
    

    Expression

    Bacterial

    Mutation

    partial genomic

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(-)-V5.hOr47a

    Plasmid

    #126474

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-opEBNA2-IRES-GFP

    Plasmid

    #220354

    Purpose

    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    3 x HA tag

    Expression

    Mammalian

    Mutation

    codon-optimised for expression in mammalian cells

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCH1/RAD51o

    Plasmid

    #102562

    Purpose

    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

    Depositor

    Maria Spies

    Article

    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

    Inserts

    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
    E. coli groE operon
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli Expression

    Promoter

    T7

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCSF-R(DD)-luc

    Plasmid

    #12424

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1994 Dec . 14(12):8085-95

    Insert

    M-CSF receptor promoter fragment (CSF1R Human)
    

    Use

    Luciferase

    Mutation

    Deletion from bp-86 to -37 (created a KpnI site i…

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1(-)-V5.mER.hOr47a

    Plasmid

    #126473

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid

    #185910

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-SOUL-P2A-tdTomato

    Plasmid

    #153019

    Purpose

    Step-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.

    Depositor

    Guoping Feng

    Article

    Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

    Insert

    SOUL
    

    Use

    AAV

    Tags

    P2A-tdTomato

    Expression

    Mammalian

    Mutation

    C128S/D156A/T159C

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

    Plasmid

    #185915

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid

    #126472

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid

    #185911

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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