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Hy_pMT Flag-URH49 MUT

Plasmid

#110126

Purpose

Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Publication

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

URH49 (DDX39A Human)

Tags

Flag

Expression

Insect

Mutation

4 amino acid mutations (RSFS motif changed to KSL…

Promoter

Metallothionein Promoter (pMT)

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E MUT

Plasmid

#110122

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Publication

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

4 mutations (KKLN motif changed to RSFS)

Promoter

Metallothionein Promoter (pMT)

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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cfSYFP2-N

Plasmid

#110631

Purpose

Cys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.

Depositor

Ikuo Wada

Insert

cfSYFP2-N

Tags

Multi-cloning sites

Expression

Mammalian

Mutation

Cys48Ser and Cys70/Met

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisA-HOXC6-2

Plasmid

#110365

Purpose

Mammalian expression of HisA-tagged HOXC6-2

Depositor

Carlos Moreno

Publication

Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

Insert

HOXC6 (HOXC6 Human)

Tags

HIS

Expression

Mammalian

Mutation

short isoform

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK224

Plasmid

#110590

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK110

Plasmid

#110576

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP6 Promoter with RBS

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK112

Plasmid

#110577

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP12b Promoter with RBS

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK113

Plasmid

#110578

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP32 Promoter with RBS

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAW8_tdTomato

Plasmid

#110239

Purpose

Cre/Lox C-terminal tagging construct encoding red fluorescent protein tdTomato

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_GST

Plasmid

#110237

Purpose

Cre/Lox C-terminal tagging construct encoding glutathione S-transferase

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_6XHis

Plasmid

#110236

Purpose

Cre/Lox C-terminal tagging construct encoding six histidine (His) residues

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_mCherry

Plasmid

#110238

Purpose

Cre/Lox C-terminal tagging construct encoding red fluorescent protein mCherry

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_13Myc

Plasmid

#110232

Purpose

Cre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUCHR-mClover-smAID-P2A-CD5Flag2-bglpA

Plasmid

#110654

Purpose

Lentiviral vector expressing cell surface Flag-tag and monomeric GFP C-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Publication

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

mClover-AID-P2A-CD5Flag2-bglpA

Use

Lentiviral

Promoter

CMV

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUCHR-mClover-smAID-P2A-CD5cmyc2-bglpA

Plasmid

#110656

Purpose

Lentiviral vector expressing cell surface c-Myc-tag and monomeric GFP C-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Publication

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

mClover-AID-P2A-CD5cmyc2-bglpA

Use

Lentiviral

Promoter

CMV

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-CD5cmyc2-bglpA

Plasmid

#110645

Purpose

Expression plasmid for c-Myc-tag embedded into human CD52 with short polyA

Depositor

Dmitriy Mazurov

Publication

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CDcmyc2-bglpA

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAW5

Plasmid

#110221

Purpose

Cre-expression plasmid

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAW1

Plasmid

#110220

Purpose

integration of loxP-ura4+-loxM3 cassette

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Mutation

See depositor comments below.

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW32

Plasmid

#110225

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW33

Plasmid

#110226

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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