We narrowed to 12,360 results for: NUC

1. pRG659_lexO-FseI_LexA-TF_LEU2MX

   Plasmid

   #154816

   Purpose

   Inducible expression of FseI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   FseI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRG646_lexO-HO_LexA-TF_LEU2MX

   Plasmid

   #154815

   Purpose

   Inducible expression of HO endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   HO endonuclease (HO Budding Yeast)
   LexA-ER-B112
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a His tag-FRB-Venus C

   Plasmid

   #100980

   Purpose

   Encodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from Bacteria

   Depositor

   Heinrich Leonhardt

   Article

   Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

   Insert

   His-FRB-Venus C
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-LBR-mRFP1-polyA

   Plasmid

   #59749

   Purpose

   Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   LBR-mRFP1
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   aa 1-249 of LBR

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPbuCas13b

   Plasmid

   #184565

   Purpose

   Inducible expression of PbuCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Cas13b nuclease from Prevotella buccae
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRG714_lexO-AscI_LexA-TF_URA3MX

   Plasmid

   #154822

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRG645_lexO-AscI_LexA-TF_LEU2MX

   Plasmid

   #154813

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGem-nHyPer2:sHyper2

   Plasmid

   #84737

   Purpose

   Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   HyPer2 targeted to nucleus
   

   Use

   Tags

   NLS-SV40

   Expression

   Plant

   Mutation

   Promoter

   pFMV/p35S

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDZ617 pKAN 12xPP7 V4

   Plasmid

   #72237

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   12xPP7 V4
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT3TS-nCas9n-A71

    Plasmid

    #125644

    Purpose

    micro-injection of poly-adenylated nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    nCas9n
    

    Use

    Rna in vitro transcription vector

    Tags

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRG710_lexO-HO_LexA-TF_HIS3MX

    Plasmid

    #154819

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-ELYS-emerin-EGFP-polyA

    Plasmid

    #59745

    Purpose

    Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    ELYS-emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDZ645 pKAN 1x-mCherry-12xPP7 V4

    Plasmid

    #73173

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    1x-mCherry
    

    Use

    Tags

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-CTE-Luc

    Plasmid

    #60881

    Purpose

    Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE

    Depositor

    Vinay Nandicoori

    Article

    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

    Insert

    Luc-CTE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pASH3 [pmyo-3::BeCNG1-YFP]

    Plasmid

    #168167

    Purpose

    Expression of BeCNG1-YFP in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    BeCNG1-YFP
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-c-Myc NLS-FKBP-Venus N-T2A-mRFP

    Plasmid

    #100979

    Purpose

    Encodes half of a protein delivery sensor ( NLS-FKBP-VN-T2A-RFP) for expression in mammalian cells

    Depositor

    Heinrich Leonhardt

    Article

    Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

    Insert

    c-Myc NLS-FKBP-Venus N-T2A-mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-(RTRG)6

    Plasmid

    #69849

    Purpose

    The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

    Depositor

    Daniel Christophe

    Article

    Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

    Insert

    6 RTRG repeats
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

    Plasmid

    #72240

    Purpose

    Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    GLT1 GAL1p 24xPP7 V3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRG711_lexO-AscI_LexA-TF_HIS3MX

    Plasmid

    #154820

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mGDF8 donor mCherry-PA

    Plasmid

    #72596

    Purpose

    Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.

    Depositor

    Renzhi Han

    Article

    Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

    Insert

    mCherry-puromycin cassetter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only