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pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP1FBAR(516a.a.)-EGFP

Plasmid

#168505

Purpose

Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP1-FBAR (SRGAP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-516

Promoter

CAG

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBEAST_J23101-bphC

Plasmid

#128137

Purpose

The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

bphC

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-BD-cop1at(335-675)

Plasmid

#44973

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

BD-COP1 (COP1 Mustard Weed)

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

deleted amino acids 1-334

Promoter

pCMV

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pAB088i

Plasmid

#79216

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAB085c

Plasmid

#79208

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ptet

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAB085d

Plasmid

#79209

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ptet

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pJKW1813

Plasmid

#174003

Purpose

Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

Depositor

Jing-Ke Weng

Article

Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

Inserts

PpCYP90G4
ATR1 (ATR1 Mustard Weed)

Expression

Yeast

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPyCAG-NE-EGFP-IRES-Pac

Plasmid

#183617

Purpose

Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

Insert

EGFP

Tags

Human EMD transmembrane domain and NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAB085d7

Plasmid

#79211

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnCAD-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAB085d6

Plasmid

#79210

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnTBR3-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pVG173

Plasmid

#251208

Purpose

Expresses a version of light-inducible transcription factor EL222 (Gly80Arg) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

Gly80Arg

Promoter

PGK1pr

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pVG298

Plasmid

#227762

Purpose

Expresses a version of light-inducible transcription factor EL222 (Thr83Ala) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Thr83Ala mutant

Promoter

PGK1pr

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTL54_pyCAG-s36GFPmNG-t2A-mCherry3NLS-polyA

Plasmid

#223546

Purpose

Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by t2A self-cleaving peptide, strong constitutive promoter CAG, no insulation or mammalian selection gene

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-mNeonGreen, mCherry-3NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pVG286

Plasmid

#227756

Purpose

Expresses a version of light-inducible transcription factor EL222 (Gly80Glu) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Gly80Glu mutant

Promoter

PGK1pr

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

Plasmid

#232128

Purpose

A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA2 (GATA2 Human)

Use

Gateway Cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#232124

Purpose

A middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Gateway Cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#232123

Purpose

A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Gateway Cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)

Plasmid

#232129

Purpose

A middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

MAFB (MAFB Human)

Use

Gateway Cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

Plasmid

#232130

Purpose

A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA4 (GATA4 Human)

Use

Gateway Cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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