Addgene

We narrowed to 4,219 results for: biorxiv

Showing: 681 - 700 of 4219 results

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PLEKHA3 (FAPP1) g1 lentiCRISPRv2-opti

Plasmid

#218656

Purpose

Knockout vector for human PLEKHA3 (FAPP1)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

PLEKHA3 (PLEKHA3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-OmpA-NHSTIIT-GG

Plasmid

#215790

Purpose

Cloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavage

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-SufI-NHSTIIT-GG

Plasmid

#215791

Purpose

Cloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavage

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC- AVITAG-CTH10STII- -GG

Plasmid

#215805

Purpose

Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-HIS6-SUMO1-AVITAG-GG

Plasmid

#215808

Purpose

Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pIA035

Plasmid

#200426

Purpose

amyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al bioRxiv 2023

Insert

LacI

Use

Tags

dronPA

Expression

Bacterial

Mutation

Promoter

Ppen

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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nTALE+CD_for TUB_8N_1397NC

Plasmid

#193383

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For multiplex C-to-T base editing to introduce amino acid mutations in the coding sequence of AtTUBs.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDESTpK7WG2-RPS5Ap-NLS-OleGFP

Plasmid

#193370

Purpose

nTALEN or nTALECD vector assembly, Destination vector for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4-R3_HSPter-RPS5A-NLS

Plasmid

#193371

Purpose

nTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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nTALECD for_CYO1 exon1_1397NC

Plasmid

#193376

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtCYO1.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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nTALECD for_CYO1 exon2_1397NC

Plasmid

#193377

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtCYO1.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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nTALECD for_PKT3 exon1_1397NC

Plasmid

#193378

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtPKT3.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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nTALECD for_PKT3 exon12_1397NC

Plasmid

#193379

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtPKT3.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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nTALECD for_MSH1 exon5_1397NC

Plasmid

#193380

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtMSH1.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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nTALECD for_MSH1 exon21_1397NC

Plasmid

#193381

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtMSH1.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK1059

Plasmid

#209549

Purpose

BTK Type 5 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv 2023.09.19.558440

Insert

Downstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLXMA L1A

Plasmid

#153347

Purpose

Expression vector for plants with medium-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-paGFP-bPAC

Plasmid

#204670

Purpose

Mammalian expression of bPAC tagged with paGFP for cAMP production

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Insert

paGFP-bPAC

Use

Lentiviral

Tags

10xHis and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)

Plasmid

#203542

Purpose

Make FIP200 NTD:ATG13:ULK1 complex

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199487

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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