We narrowed to 3,609 results for: golden gate

1. pICH87644

   Plasmid

   #50274

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter, act2 (AT3G18780, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAGM1479

   Plasmid

   #50291

   Depositor

   Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR, omega (Tobacco Mosaic Virus) + N terminal HIS tag (6x polyhistidine)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAGM5331

   Plasmid

   #50294

   Depositor

   Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR, omega (Tobacco Mosaic Virus) + nuclear localisation signal (Simian Virus 40)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAGM1482

   Plasmid

   #50295

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR, omega (Tobacco Mosaic Virus) + mitochondrial localisation signal ScCoxIV (Saccharomyces cerevisiae)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pICH45195

   Plasmid

   #50275

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter + 5' UTR, RbcS2B (AT5g38420, A. thaliana)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICH85281

   Plasmid

   #50272

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   Promoter + 5'UTR, mas, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAGM5343

   Plasmid

   #50297

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (O. sativa) + HIS tag
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C-term_aMFx1 (4a)

   Plasmid

   #238900

   Purpose

   Plasmid insert contains the aMFx1 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   C-term_aMFx1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C-term_aMFx1_noKREAEA (4a)

   Plasmid

   #238901

   Purpose

   Plasmid insert contains the aMFx1 noKREAEA C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   C-term_aMFx1_noKREAEA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C-term_aMFx4 (4a)

    Plasmid

    #238902

    Purpose

    Plasmid insert contains the aMFx4 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_aMFx4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. twinStrep_FLAG_aMFx1 (4a)

    Plasmid

    #238904

    Purpose

    Plasmid insert contains the aMFx1, FLAG and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    twinStrep_FLAG_aMFx1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. twinStrep_HA_aMFx1 (4a)

    Plasmid

    #238905

    Purpose

    Plasmid insert contains the aMFx1, HA and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    twinStrep_HA_aMFx1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. twinStrep_aMFx1 (4a)

    Plasmid

    #238906

    Purpose

    Plasmid insert contains the aMFx1 and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    twinStrep_aMFx1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBD_PYR1_156

    Plasmid

    #241285

    Purpose

    Backbone for PYR1 sensor library assembly from oligopools; domesticated for golden gate cloning

    Depositor

    Sean Cutler

    Article

    Tian et al bioRxiv [Preprint]. 2025 May 15:2025.05.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBTK1129

    Plasmid

    #236752

    Purpose

    BTK Type 2-3 fluorescent protein cassette for golden gate assembly. Improved version of pBTK1055

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al mBio. 2024 Sep 11;15(9):e0139224. doi: 1

    Insert

    Type 2-3 GFP cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    CP25

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YEE_GFP

    Plasmid

    #228184

    Purpose

    Yeast episomal expression vector with type '234' GFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YEE_RFP

    Plasmid

    #228185

    Purpose

    Yeast episomal expression vector with type '234' RFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKEK3399

    Plasmid

    #234083

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    DasherGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKEK3225

    Plasmid

    #234087

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    DasherGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKEK3396

    Plasmid

    #234080

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    aeBlue
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge