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Plasmid#215264PurposeModule for the expression of HISPS under the ed light regulated Etr8:CMVmin promoter and H3H under the copper inducible CBS:mDFR promoter and the constitutive expression of LUZ, CPH,EGFP and p19DepositorInsertEtr4:mDFR:HISPS-CBS4x:mDFR:H3HSF-LUZ-CPH-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4541
Plasmid#215260PurposeModule for the expression of the N. nambi HISPS gene under the regulation of the Red light-regulated Etr4:mDFR promoter and TNOS in and the constitutve expression of CPH, LUZ, H3H, EGFP and p19.DepositorInsertEtr4:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:AsCas12a_DR (GB1442)
Plasmid#160574PurposeAsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:AsCas12a_DR
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 LbCas12a (GB1439)
Plasmid#160573PurposeHuman codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).DepositorInsertLbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AsCas12a (GB1438)
Plasmid#160572PurposeHuman codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).DepositorInsertAsCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC45
Plasmid#104819PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC29
Plasmid#104803PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr4g094545
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C.S61P
Plasmid#105048PurposeOverexpression of APOBEC3C S61P mutant in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationserine 61 to prolinePromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C.D99E
Plasmid#105049PurposeOverexpression of APOBEC3C D99E mutant in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationAspartate 99 to glutamatePromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C.DS-EP
Plasmid#105050PurposeOverexpression of APOBEC3C DS-EP mutant in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationS61P, D99EPromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C.C97S
Plasmid#105051PurposeOverexpression of APOBEC3C C97S mutant in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationcysteine 97 to serinePromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-PM
Plasmid#183168PurposeConstruct for transient expression of plasma membrane-targeted mCherry in plants.DepositorInsertmCherry-PM
UseTagsOsRac3, O. sativa rac-like GTP-binding protein 3ExpressionPlantMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGT8163 (AATG_ttLbCas12a-i_TTCG)
Plasmid#192453Purposeintronized ttLbCas12a for in planta expressionDepositorInserttemperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
UseCRISPR and Synthetic Biology; Moclo compatible le…TagsExpressionMutationtemperature-tolerant mutation (D156R)PromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/NLS-mChe
Plasmid#183162PurposeConstruct for transient expression of cell nucleus-targeted mCherry in plants.DepositorInsertNLS-mCherry
UseTags16 aa nuclear localization signal and 16 aa nucl…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEL032
Plasmid#137901PurposeSTU2.0 LbCas12a for plant genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166
Plasmid#109328PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/ER-mChe
Plasmid#183163PurposeConstruct for transient expression of endoplasmic reticulum-targeted mCherry in plants.DepositorInsertER-mCherry
UseTags25 aa ER signal of Glycine soja kunitz-type tryps…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3C-BE3
Plasmid#113412PurposeExpresses hA3C-BE3 in mammalian cellsDepositorInserthA3C-BE3 (APOBEC3C S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-FIT2
Plasmid#96990PurposeExpress mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:LbCas12a_DR (GB1443)
Plasmid#160575PurposeLbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:LbCas12a_DR
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterU6-26Available sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only