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pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)
Plasmid#185706PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLSDepositorInsertCas9
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)
Plasmid#185701PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(FCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)
Plasmid#185705PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(PDCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)
Plasmid#185703PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(SCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)
Plasmid#185704PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(PCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)
Plasmid#185702PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(BCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaoA
Plasmid#196051PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensorDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
hHSF4b K294R
Plasmid#118357PurposeThis plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationK294RPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)
Plasmid#185488PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)
Plasmid#185485PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)
Plasmid#185484PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP (LTH1441)
Plasmid#185486PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(PCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-EGFP (LTH1380)
Plasmid#185487PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSOD1-FLAG
Plasmid#182922PurposeMammalian expression plasmid for human WT SOD1DepositorInsert[Cu-Zn] Superoxide dismutase 1 (SOD1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
hMEF2A K403R
Plasmid#118355PurposeThis plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.DepositorInserthMEF2A (MEF2A Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK403RPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
GANLS-DEVD-BNES
Plasmid#50835PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddGFP A and ddRFP B
UseTagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
GANES-DEVD-BNLS
Plasmid#50842PurposeExpresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.DepositorInsertddGFP A and ddGFP B
UseTagsNES sequence LALKLAGLDIGS placed after ddGFP A an…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
NESRA-DEVD-BNLS
Plasmid#50849PurposeExpresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.DepositorInsertddRFP A and ddRFP B
UseTagsNES sequence LQKKLEELELDE placed after ddGFP A an…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only
