We narrowed to 171,035 results for: addgene

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KLF17-CRa2-Lsg-MS2

Plasmid

#247477

Purpose

gRNA2 for KLF17 activation cloned into LsgRNA-MS2 backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA2 targeting KLF17 promoter (KLF17 Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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D10A-Cas9

Plasmid

#239383

Purpose

For circular RNA-mediated inverse prime editor using nCas9-D10A in HEK294T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

nCas9-D10A

Use

CRISPR

Tags

BPNLS and SV40 NLS

Expression

Mammalian

Mutation

D10A in Cas9

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-1

Plasmid

#200539

Purpose

mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-2

Plasmid

#200540

Purpose

mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-3

Plasmid

#200541

Purpose

mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_CreER-pA-FRT-acry:venus-pA-FRT

Plasmid

#200543

Purpose

mini circle middle entry plasmid for CreER-pA-FRT-acry:venus-pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_CreER-pA-FRT-acry:mCherry-pA-FRT

Plasmid

#200544

Purpose

mini circle middle entry plasmid for CreER-pA-FRT-acry: mCherry -pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER, mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-1 without ATG

Plasmid

#200525

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 1 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-2 without ATG

Plasmid

#200526

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 2 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-3 without ATG

Plasmid

#200527

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 3 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-1

Plasmid

#200529

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-2

Plasmid

#200530

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-mCherry-P2A-NTRv2-pA ORF-3

Plasmid

#200531

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-BFP-pA ORF-1 without ATG

Plasmid

#200534

Purpose

mini circle middle entry plasmid for 2a-BFP-pA ORF 1 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-BFP-pA ORF-2 without ATG

Plasmid

#200535

Purpose

mini circle middle entry plasmid for 2a-BFP-pA ORF 2 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-BFP-pA ORF-3 without ATG

Plasmid

#200536

Purpose

mini circle middle entry plasmid for 2a-BFP-pA ORF 3 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM32

Plasmid

#233504

Purpose

Level 0 plasmid for MoClo, encodes inducible PGK1-tetO2p for K. marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

PGK1-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM02

Plasmid

#233474

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Nat selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM04

Plasmid

#233476

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-G418 selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM06

Plasmid

#233478

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Hyg selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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