We narrowed to 10,147 results for: gnas

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pCDH-3_Flag-YTHDF2-D-MUT

Plasmid

#238977

Purpose

Expresses the domain-mutant YTHDF2

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2-D-MUT (YTHDF2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

5 site mutation (W432A+W486A+Y418A+D422A+W491A)

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE

Plasmid

#236241

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV1.2 (CACNA1C Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-Sec61b WPRE

Plasmid

#236229

Purpose

AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

HaloTag-Sec61b (SEC61B Human, Synthetic)

Use

AAV

Tags

HaloTag

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

Plasmid

#236245

Purpose

Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

U6 and human Synapsin1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

Plasmid

#236240

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV2.2 (CACNA1B Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTR01F/hFGFR3b-V5

Plasmid

#236016

Purpose

for PiggyBac mediated integration and stable expression of hFGFR3b protein

Depositor

Pavel Krejčí

Article

Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

Insert

human FGFR3b (FGFR3 Human)

Tags

V5/His

Expression

Mammalian

Promoter

truncated CMV promoter

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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MKC131_CCR5(SFFV-synEPOR-2A-YFP)

Plasmid

#232412

Purpose

AAV production plasmid for SFFV(synEPOR) vector from Figs. 2-3 that mediates HDR at CCR5 locus using CCR5 gRNA. YFP is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.

Depositor

Kyle Cromer

Article

Shah et al Nat Commun. 2025 Jan 29;16(1):1140. doi:

Insert

Truncated Erythropoietin Receptor (EPOR Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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MKC175_HBA1reg(synEPOR)

Plasmid

#232413

Purpose

AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank H+C15BA1 cassette. HAs are ~400bp each.

Depositor

Kyle Cromer

Article

Shah et al Nat Commun. 2025 Jan 29;16(1):1140. doi:

Insert

Truncated Erythropoietin Receptor (EPOR Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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STIM1-RspA-NFAST

Plasmid

#233606

Purpose

Expression of hSTIM1-RspA-NFAST

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

STIM1 (STIM1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R82A_pET-14b

Plasmid

#233276

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

Depositor

Mohan Babu, Andrew Emili

Article

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 82…

Promoter

T7 promoter

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R64A_pET-14b

Plasmid

#233263

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

Depositor

Mohan Babu, Andrew Emili

Article

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 64…

Promoter

T7 promoter

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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MKC177_CCR5(PGK-synEPOR)

Plasmid

#232415

Purpose

AAV production plasmid for PGK(synEPOR) vector from Figs. 3-5 that mediates HDR at CCR5 locus using CCR5 gRNA. synEPOR is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.

Depositor

Kyle Cromer

Article

Shah et al Nat Commun. 2025 Jan 29;16(1):1140. doi:

Insert

Truncated Erythropoietin Receptor (EPOR Human)

Use

AAV

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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HemH-R212A_pET-14b

Plasmid

#233262

Purpose

Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

Depositor

Mohan Babu, Andrew Emili

Article

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

HemH (hemH )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 21…

Promoter

T7 promoter

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbleo-TGFa-EREG-ScNeo

Plasmid

#209915

Purpose

To express the chimeric protein of TGFα and EREG, which a recombinant TGFα protein fused extracellularly to the mScarlet and a recombinant Epiregulin protein fused intracellularly to the mNeonGreen

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-EREG-ScNeo (EREG, TGFA Human)

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag-RBD Omicron XBB.1.5

Plasmid

#214724

Purpose

Expresses SARS-CoV-2 Omicron XBB.1.5 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Tags

SpyTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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CILK1-R272Q

Plasmid

#214911

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Tags

Myc-FLAG

Expression

Mammalian

Mutation

R272Q substitution

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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CILK1-E80K

Plasmid

#214910

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Tags

Myc-FLAG

Expression

Mammalian

Mutation

E80K substitution

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO shLuc/FLAG Metap2

Plasmid

#209316

Purpose

Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

METAP2 (METAP2 Human, human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO shLYCHOS/LYCHOS WT-FLAG

Plasmid

#209315

Purpose

Lentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO shLYCHOS/FLAG Metap2

Plasmid

#209314

Purpose

Lentiviral construct expressing FLAG Metap2 and shRNA against LYCHOS

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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