We narrowed to 8,493 results for: sars

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pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

Plasmid

#87803

Purpose

Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

NLS
PTS1

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEntr_Myc-BirA-G3-ER

Plasmid

#173814

Purpose

Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

Depositor

Norbert Perrimon

Article

Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

Insert

Myc-BirA-G3-ER

Use

Gateway shuttle vector

Tags

Myc

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI194

Plasmid

#84266

Purpose

Expresses Avi-tagged RPL1 and humanized birA

Depositor

Nicholas Ingolia

Article

Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

Inserts

RPL1 (RPL10A Human, (*see below))
humanized birA

Tags

Avi tag, Flag, Gly/Ser linker, and TEV site

Expression

Mammalian

Mutation

R33A, A34E (*see below)

Promoter

CMV and P2A peptide

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A-L325R

Plasmid

#145373

Purpose

expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

Depositor

Hugues Abriel

Article

Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Expression

Mammalian

Mutation

changed Leucine 325 to Arginine

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

Plasmid

#154774

Purpose

plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

in PylT: UUA anticodon and G10A, G14U, G16U, C20U…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mGSDME

Plasmid

#154877

Purpose

Expresses mGSDME in mammalian cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (Gsdme Mouse)

Use

Lentiviral

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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MCP-3x9aaTAD

Plasmid

#210707

Purpose

This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

N55K in MCP

Promoter

EF1a

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-CD8aStalk-PDGFR

Plasmid

#240242

Purpose

Lentiviral transfer plasmid encoding IL13 with CD8a stalk and PDGFR TM domain

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mRuby3-IRES-puro

Plasmid

#85144

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)

Use

Lentiviral

Tags

mRuby3

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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HA-KIF5B*-strep

Plasmid

#120170

Purpose

Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-human-CHMP3

Plasmid

#154180

Purpose

expresses human CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (CHMP3 Human)

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-Ef1a-SOX2-3xFLAG

Plasmid

#216197

Purpose

Overexpress human SOX2 in HEK 293T cells for use in whole cell extract EMSAs

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX2 (SOX2 Human)

Use

Lentiviral

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

Plasmid

#162386

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xSTREP Cyclin F(LP35/36AA)

Plasmid

#236467

Purpose

transient overexpression of Cyclin F in mammalian cells

Depositor

Michele Pagano

Article

D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

Insert

Cyclin F (CCNF Human)

Tags

2xSTREP

Expression

Mammalian

Mutation

(LP35/36AA) first two amino acids of the F-box do…

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-IgG4hinge-PDGFR

Plasmid

#240245

Purpose

Lentiviral transfer plasmid encoding IL13 with IgG4 hinge and PDGFR TM domain

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shSTING-Mmu

Plasmid

#127697

Purpose

Doxycyclin inducible shRNA knockdown of mouse STING gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shSTING Mmu (Sting1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-CHC(1-1074)-His6

Plasmid

#100748

Purpose

Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CLTC (CLTC Human)

Tags

His and MBP

Expression

Bacterial

Mutation

Residues 1-1074

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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aav-tnt-wtmrtfa-gfp

Plasmid

#165037

Purpose

AAV-based delivery of wtMRTFA-GFP into cardiomyocytes in vivo

Depositor

William Pu

Article

Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

Mrtfa (Mrtfa Mouse)

Use

AAV

Tags

GFP

Promoter

cTNT

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMEXneo-TrkB

Plasmid

#190203

Purpose

To express the Human TrkB receptor under the Moloney mouse sarcoma virus LTR

Depositor

Pantelis Tsoulfas

Article

Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

Insert

TrkB (NTRK2 Human)

Expression

Mammalian

Promoter

Moloney mouse sarcoma virus right LTR

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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PEX3*-SBP-GFP

Plasmid

#120174

Purpose

Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Peroxisomal biogenesis factor 3 (PEX3 Human)

Tags

SBP and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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