We narrowed to 8,324 results for: gnal

1. pcDNA3-myc-mCE NLS

   Plasmid

   #82465

   Purpose

   Expresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   Capping Enzyme (Rngtt Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   deleted amino acids Nuclear Localization Signal

   Promoter

   CMV

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-myc NES-mCE

   Plasmid

   #82463

   Purpose

   Expression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   NES mRNA capping enzyme (Rngtt Mouse, HIV)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCellFree_G03 STAT1

   Plasmid

   #67116

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   STAT1 (STAT1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pYNL-C1_PTS1

   Plasmid

   #65699

   Purpose

   Expresses YNL-PTS1 in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 1 (tripeptide SKL)
   

   Use

   Luciferase

   Tags

   YNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCNL-C1_PTS1

   Plasmid

   #65700

   Purpose

   Expresses CNL-PTS1 in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 1 (tripeptide SKL)
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pONL-C1_PTS1

   Plasmid

   #65701

   Purpose

   Expresses ONL-PTS1 in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 1 (tripeptide SKL)
   

   Use

   Luciferase

   Tags

   ONL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcdna3 EE Jip1b

   Plasmid

   #52127

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
   

   Use

   Tags

   EE

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 18, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pET21-MRPL13

   Plasmid

   #31344

   Depositor

   Linda Spremulli

   Article

   Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

   Insert

   mitochondrial ribosomal protein L13 mature form (MRPL13 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Mature form after removal of the predicted mitoch…

   Promoter

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pCeSR beta deltaN

   Plasmid

   #13728

   Depositor

   Thomas Schwartz

   Article

   Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

   Insert

   Signal recognition particle receptor β subunit (R186.3 Nematode)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   expresses residues 38-240 of C. elegans SRβ with …

   Promoter

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScSR beta deltaN Flag

    Plasmid

    #13729

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit (SRP102 Budding Yeast)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    expresses residues 31-244 of S. cerevisiae SRβ wi…

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mCherry-PKCsub-RLuc8N-NES

    Plasmid

    #138213

    Purpose

    Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    mCherry-PKCsub-RLuc8N-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

    Plasmid

    #207664

    Purpose

    Neuropeptide degradation

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    NEP (MME Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mRUBY3

    Expression

    Mutation

    Addition of signaling peptide (POMC) on the N-ter…

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTAMAHISTEV_PfeA

    Plasmid

    #128943

    Purpose

    expresses PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid

    #111925

    Purpose

    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. anti-FLAG M2 heavy chain

    Plasmid

    #175359

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 heavy chain
    

    Use

    Tags

    His8 tag and N-terminal secretion signal peptide …

    Expression

    Mammalian

    Mutation

    L51I (see paper)

    Promoter

    CMV

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS413-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221095

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. YAP/TAZ tdTomato TEAD Binding Reporter

    Plasmid

    #228554

    Purpose

    Encodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.

    Depositor

    Cole DeForest

    Article

    Kopyeva et al Adv Mater. 2024 Sep 6:e2404880. doi: 10.

    Insert

    tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GTIIC TEAD-Binding Responsive Element

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-fDIO-mem-seTurboID-IRES-tTA

    Plasmid

    #237889

    Purpose

    Amplifier AAV vector of Dual-AAV sparse labeling system encoding membrane-tethered seTurboID

    Depositor

    Rui Lin

    Article

    Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

    Insert

    mem-seTurboID
    

    Use

    AAV

    Tags

    FLAG tag and GAP43 palmitoylation signal

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-DIO-mem-seTurboID

    Plasmid

    #237887

    Purpose

    AAV vector for Cre-dependent expression of membrane-tethered seTurboID

    Depositor

    Rui Lin

    Article

    Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

    Insert

    mem-seTurboID
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG tag and GAP43 palmitoylation signal

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-myc-HLA-A3

    Plasmid

    #135505

    Purpose

    Mammalian expression of myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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