We narrowed to 7,234 results for: CAR
-
Plasmid#166807PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pcDNA 3.1 LIC 6A NLRP1-FLAG P1214R
Plasmid#166806PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The P1214R patient mutant abolishes NLRP1-CT association with DPP9 at interface II.DepositorAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
dTAG NLRP1 P1214R
Plasmid#166826PurposedTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradationDepositorAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
prCOrev
Plasmid#153961PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCO
Plasmid#153960PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)
Plasmid#155136PurposeExpresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1DepositorInsertGlpG (glpG Escherichia coli)
TagsGST and HAExpressionBacterialMutationD243G/L244G/F245G/M247G/S248G/M249G/A250GPromotertacAvailable sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
rd
Plasmid#112912PurposeExpress the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tagDepositorInsertdematin (DMTN Human)
TagsGlutatione-S-Tranferase and precision protease si…ExpressionBacterialMutationPEST sequence near the N-terminal removed. 5′-GCG…PromoterT7Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]
Plasmid#106462PurposeExpresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W(N163,242Q)
TagsPAExpressionBacterial and YeastMutationchanged Asparagines at positions 163 and 242 to G…PromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]
Plasmid#106466PurposeExpresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(S219A)
TagsGFPExpressionBacterial and YeastMutationChanged Serine 219 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]
Plasmid#106467PurposeExpresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(H474A)
TagsGFPExpressionBacterial and YeastMutationChanged Histidine 474 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]
Plasmid#106468PurposeExpresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(D415A)
TagsGFPExpressionBacterial and YeastMutationChanged Aspartate 415 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3 SIRT1-NTerm Exon1-Exon3
Plasmid#105681PurposeBacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)DepositorInsertSIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)
TagsGSTExpressionBacterialMutationSynonymous base changes not affecting the protein…Promotertac_PromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3
Plasmid#105680PurposeBacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3DepositorInsertSIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)
TagsGSTExpressionBacterialMutationSynonymous base changes not affecting the protein…Promotertac_PromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-RP2-C86Y,P95L
Plasmid#86074PurposeA bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.DepositorInsertRP2 (RP2 Human)
TagsGSTExpressionBacterialMutationChanged cysteine-86 and proline-95 to tyrosine an…PromotertacAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shDUSP19 puro
Plasmid#87796PurposeExpresses an inducible short hairpin targeting human DUSP19 sequenceDepositorAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP7.v2 puro
Plasmid#87795PurposeExpresses an inducible short hairpin targeting human MKP7 sequenceDepositorAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 D102A, E108A
Plasmid#74618PurposeExpresses a double mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 N68A, W69A, K77A
Plasmid#74619PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialPromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
SHPK gRNA (BRDN0001149277)
Plasmid#77217Purpose3rd generation lentiviral gRNA plasmid targeting human SHPKDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SHPK gRNA (BRDN0001146114)
Plasmid#77218Purpose3rd generation lentiviral gRNA plasmid targeting human SHPKDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only