164,722 results

1. pMaster12

   Plasmid

   #58527

   Purpose

   Reprogram somatic cells to iPS cells

   Depositor

   Mario Capecchi

   Article

   Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

   Inserts

   neo
   tk
   oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 Human)
   Myc (MYC Human)
   EBNA-1
   Amp
   Lin28 (LIN28A Human)
   NANOG (NANOG Human)
   IRES
   NR5A2 (NR5A2 Human)
   miR302/367 (MIR367, MIR302A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRT801

   Plasmid

   #68112

   Purpose

   Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

   Depositor

   Maggie Smith

   Article

   Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

   Inserts

   aac3(IV)
   oriT
   attP
   int
   

   Use

   Streptomyces integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pgRNA-bacteria

   Plasmid

   #44251

   Purpose

   Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA (bacteria)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 (SpeI)

   Available Since

   April 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 c-SRC (WT)

   Plasmid

   #42202

   Depositor

   Robert Lefkowitz

   Article

   Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

   Insert

   c-SRC (SRC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L176F mutation compared to reference sequence NM_…

   Promoter

   CMV

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGE503

   Plasmid

   #153276

   Purpose

   Intermediate sgRNA array cloning vector position 1

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGE504

   Plasmid

   #153277

   Purpose

   Intermediate sgRNA array cloning vector position 2

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGE508

   Plasmid

   #153280

   Purpose

   End-linker for cloning 2 sgRNA arrays into any recipient vector

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Insert

   linker
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRM210 LGP (Luciferase GFP Puro)

   Plasmid

   #174722

   Purpose

   Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   eGFP
   pac
   

   Use

   Lentiviral and Luciferase

   Tags

   E2A linker and F2A linker

   Expression

   Mammalian

   Mutation

   Promoter

   MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.LSL.tdTomato

   Plasmid

   #100048

   Purpose

   AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdtomato
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9_GFP

    Plasmid

    #44719

    Purpose

    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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