We narrowed to 18,671 results for: Met;

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pBHM2680

Plasmid

#241725

Purpose

Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSR marker for selection on blasticidin S

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMSP1E2D1

Plasmid

#161606

Purpose

Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E2D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mNeptune2.5

Plasmid

#197197

Purpose

Expresses the protein of mNeptune2.5 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mNeptune2.5

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1243 NES-ECFP-FIREmate

Plasmid

#205169

Purpose

Expresses NES-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NES-ECFP-FIREmate

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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mTB3-BC2 nanobody

Plasmid

#210208

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

BC2 Nanobody

Expression

Bacterial

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-rm_gCarvi-T2A

Plasmid

#190334

Purpose

Ratiometric reporter for cAMP

Depositor

Naoto Saitoh

Article

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

ratiometric gCarvi

Use

Unspecified

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAG1237 FIREmate-ECFP-Fis1

Plasmid

#205173

Purpose

Expresses FIREmate-ECFP-Fis1 in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Fis1

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRVL-4

Plasmid

#104582

Purpose

Contains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human CL kappa

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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PCT62

Plasmid

#170391

Purpose

expresses three sgRNA cassettes

Depositor

Jonathan Weissman

Article

Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

Insert

PCT62-targetsite

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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phND2-N106

Plasmid

#31780

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

NeuroD2 (NEUROD2 Human)

Use

Lentiviral

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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BFP dest clone

Plasmid

#71825

Purpose

Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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RINS1

Plasmid

#107290

Purpose

Plasmid for the expression of the RINS1 sensor

Depositor

Dmytro Yushchenko

Article

Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

Insert

RINS1

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-RLuc-T2A-PuroR

Plasmid

#102320

Purpose

expression of r-luciferase

Depositor

Rudolf Jaenisch

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Renilla luciferase

Use

Lentiviral

Promoter

Ubc

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Blast mouse E-cadherin

Plasmid

#18804

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin (Cdh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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pUC-hrDNA-12.0

Plasmid

#206320

Purpose

A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)

Depositor

Brian McStay

Article

van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1

Insert

Intergenic spacer of human rDNA repeat

Use

Cloning

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRN12

Plasmid

#84456

Purpose

shuttle plasmid for sarA P1-mAmetrine expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mAmetrine

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-8XMS2

Plasmid

#121939

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3L

Plasmid

#35523

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3L (DNMT3L Human)

Tags

Myc

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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pJKR-O-mphR

Plasmid

#62570

Purpose

Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

mCherry

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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MCP-EGFP-PiggyBac

Plasmid

#190003

Purpose

Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

Depositor

Tineke Lenstra

Article

Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

Insert

MCP-EGFP

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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