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Showing: 10591 - 10620 of 13600 results

  9. pNOC_dCas9_sgRNA Td2

    Plasmid
    #176258
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176258-plasmid-map-sequence-id-347041

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  11. pNOC_dfnCas12a_crRNA Td2

    Plasmid
    #176255
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176255-plasmid-map-sequence-id-347038

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  12. pNOC_hfnCas12a-Nlux_crRNA NR2

    Plasmid
    #176245
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176245-plasmid-map-sequence-id-346567

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  15. pNOC_hfnCas12a-Nlux_crRNA NR3

    Plasmid
    #176246
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176246-plasmid-map-sequence-id-346569

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  16. pDONR207-MUM2sp-Citrine-ADPG2

    Plasmid
    #170725
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    Use
    Gateway donor vector / entry clone
    Tags
    Citrine tag (714bp)
    Available Since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    170725-plasmid-map-sequence-id-345738

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  18. pUPD2 Register 1/3 Tnos:YFP:5'UTR (GB1507)

    Plasmid
    #160583
    Purpose
    Part 1/3 for assembly of site-specific recombination based Registers. Composed of the reversed sequences of the TNos terminator,YFP CDS and 5' UTR of 35S promoter (TNos:YFP:5'UTR)
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    YFP
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    160583-plasmid-map-sequence-id-338464

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  24. p35S_EVDm1

    Plasmid
    #167121
    Purpose
    Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding site mutated under CaMV 35S promoter.
    Depositor
    Arturo Marí-Ordóñez, Olivier Voinnet
    Article
    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do
    Insert
    EVADE (EVD) (AT5G17125 Mustard Weed)
    Expression
    Plant
    Mutation
    mutated four nucleotides at the snoRNA U1 binding…
    Promoter
    CaMV 35S
    Available Since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    167121-plasmid-map-sequence-id-333657

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  27. p35S_GFP-EVDinter-GUS

    Plasmid
    #167123
    Purpose
    Plant binary expression vector containing the intron and proximal polyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.
    Depositor
    Arturo Marí-Ordóñez, Olivier Voinnet
    Article
    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do
    Insert
    EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
    Tags
    GUS and mGFP5
    Expression
    Plant
    Promoter
    CaMV 35S
    Available Since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    167123-plasmid-map-sequence-id-331746

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  28. pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

    Plasmid
    #166855
    Purpose
    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi
    Insert
    Atg13[571–700] (ATG13 Budding Yeast)
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    K683E, H685E, K686E
    Promoter
    pCu
    Available Since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166855-plasmid-map-sequence-id-329426

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  30. pCAG-EGxxFP-CD55

    Plasmid
    #153959
    Purpose
    Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
    Use
    A plasmid specific for egxxfp assay
    Tags
    N/A
    Expression
    Mammalian
    Mutation
    No mutation
    Promoter
    CAG promoter
    Available Since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    153959-plasmid-map-sequence-id-319276

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Showing: 10591 - 10620 of 13600 results