We narrowed to 9,938 results for: Pol
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Plasmid#227226PurposeExpresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationaa 62-205PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)1x
Plasmid#112055PurposeACTB mRNA tagged with 1 copy of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
TagsRiboglow RNA tagExpressionMammalianMutation1 copy of Riboglow tag (variant A) after stop cod…PromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)
Plasmid#208976PurposeNickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.DepositorInsertnSpCas9(H840)-BPNLS-P2A-GFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A)PromoterCMV and T7Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Flag-MFF-WT
Plasmid#74379PurposeLentiviral expression vector containing flag tagged WT MFF isoform 5DepositorAvailable SinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_775-1102 USP7
Plasmid#131250PurposeMammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tagDepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationEncodes USP7 UBL domains 3-5 (USP7 amino acids 77…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-SLEV_NS2B-GS-NS3
Plasmid#203510PurposeExpresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 markerDepositorAvailable SinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn2delBRCT
Plasmid#173939PurposeBacterial expression of PARP1 lacking Zn2 and BRCT domainsDepositorInsertPARP1delZn2delBRCT (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 97-213 and 374-520; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-EXO1b-D173A
Plasmid#111622PurposeExpression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cellsDepositorAvailable SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS-mEmerald-VIM
Plasmid#62849PurposeDonor vector for gene editing at human VIM locusDepositorInsertmEmerald-VIM (VIM Human, Synthetic)
UseYeast-e.coli shuttle vectorAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo
Plasmid#212821PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cellsDepositorAvailable SinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7187
Plasmid#219761PurposeMoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)DepositorInsertPpASCL
ExpressionPlantPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCJ207
Plasmid#162680PurposepET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-extGluc-NGFR
Plasmid#104832PurposeextGluc luciferase for invivo Imaging, NGFR for flourescent detection or separationDepositorInsertsextGLuc
NGFR
UseLentiviral and LuciferaseExpressionMammalianAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTon1(il11)
Plasmid#233647PurposeExpression vector of X. laevis il11.L-P2A-AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.DepositorInsertsinterleukin-11.L (350-913) (il11.L Frog)
target sequence of guide RNA #tyr (3905-3927) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
UseAmphibian expression, tet-onMutationSome synonymous substitutions were introduced in…Available SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_Sik3_T142Q
Plasmid#119973PurposeLentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance geneDepositorInsertSik3 (Sik3 Mouse)
UseLentiviralExpressionMammalianMutationchange Threonine 142 to GlutaminePromoterEFS promoterAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EPOP
Plasmid#125170PurposeExpresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
mouse a2b full length
Plasmid#217812PurposeFull length mouse integrin a2b expressionDepositorAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-7Q
Plasmid#84347PurposeExpression of the human Huntingtin Exon1 protein containing 7Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal His6-tagged intein SspExpressionBacterialPromoterT7Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT-L713F
Plasmid#173944PurposeBacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutationDepositorAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.dEZH2
Plasmid#220241PurposeExpression of an EZH2 catalytic site mutant in insect cells.DepositorAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only