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pZ8-P_dCas9

Plasmid

#74063

Purpose

pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

dcas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Propionate inducible promoter (prp)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBSJL1

Plasmid

#71261

Purpose

E. coli-Veillonella shuttle plasmid

Depositor

Felicia Qi

Article

Liu et al Appl Environ Microbiol. 2012 May;78(9):3

Type

Empty backbone

Use

E. coli-veillonella shuttle plasmid

Expression

Bacterial

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only
pLV-tetO-Ets2

Plasmid

#70272

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ets2 (Ets2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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PNCA-GFP

Plasmid

#67789

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

Insert

MMLV-LTR-GFP

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pBSKΔB-TRE-dCas9-EGFP

Plasmid

#62347

Purpose

Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

dCas9-EGFP

Use

CRISPR

Tags

HA and SV40 NLS

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn2beta AS4(-)

Plasmid

#58268

Purpose

Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn2beta AS4(-) (Nrxn2 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_8

Plasmid

#60579

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_8

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-CGGA-GTGC

Plasmid

#60950

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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PuroR-P2A-DD-TCOF1-donor

Plasmid

#60747

Purpose

Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

Depositor

Benhur Lee

Article

Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

Insert

TCOF1 (TCOF1 Human)

Use

Targeting donor

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pLX304-YAP1 _SH3bm

Plasmid

#59141

Purpose

YAP1 mutants that harbored a deletion of SH3 binding motif, which disrupts interaction of YES1 with YAP1

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

SH3 domain deleted (aa278-290)

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
N-Terminal Split Cas9 D10A Nickase with GyrA intein

Plasmid

#58695

Purpose

Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production

Depositor

Gang Bao

Article

Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

Insert

D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein

Use

AAV and CRISPR

Tags

GyrA Nsplit Intein, HA Tag, and NLS

Expression

Mammalian

Mutation

D10A nickase converting mutation to SpCas9

Promoter

CBh

Available Since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAdSh.U6.gRNAS1

Plasmid

#58252

Purpose

U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAS1 cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV3FLAG_hGTPBP4

Plasmid

#53974

Purpose

Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

GTPBP4 (GTPBP4 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCCL-ReDNAP (Y427A)

Plasmid

#52346

Purpose

CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

Recoded TP-DNAP

Expression

Bacterial and Yeast

Promoter

REV1

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGZ003

Plasmid

#48869

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

Plasmid

#47870

Purpose

All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA
OCT4 crRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pSCT-GAL93-NONO

Plasmid

#46325

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

NONO (NONO Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only
pSCT-GAL93-PSPC1

Plasmid

#46321

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

PSPC1 (PSPC1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only
pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)

Plasmid

#44095

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

3xFlag, 6xGly linker, ADH1 transcription terminat…

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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TAL2384

Plasmid

#36793

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BRCA1-TALEN_Left (Brca1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only