We narrowed to 26,125 results for: expression vector

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pMC-6-AmCyan-P2A-NptII-9

Plasmid

#197742

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AmCyan-P2A-NptII bicistronic CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-NLSlacZ-stop-L2

Plasmid

#186358

Purpose

Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ Synthetic, E. coli)

Use

Expression of nuclear lacz reporter

Tags

Nuclear localization signal

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pgRNA_CV2

Plasmid

#167165

Purpose

pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

Depositor

Alexander Loewer

Article

Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX_307

Plasmid

#41392

Purpose

Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-Hygro-3xFLAG

Plasmid

#185359

Purpose

Vector backbone for mammalian expression of proteins with an N-terminal 3xFLAG tag.

Depositor

Ayaz Najafov

Insert

3xFLAG

Tags

3xFLAG

Expression

Mammalian

Mutation

WT

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ H2B-mTagBFP2

Plasmid

#99267

Purpose

Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Philipp Keller

Insert

mTagBFP2-3XHA

Tags

H2B

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+ H2B-mCerulean

Plasmid

#99268

Purpose

Contains a fusion of human histone H2B to fluorescent protein mCerulean in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription

Depositor

Philipp Keller

Insert

mCerulean-3XFLAG

Tags

H2B

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIPZ (M)-HT-NLS

Plasmid

#82518

Purpose

Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.

Depositor

Xiaojun Ren

Insert

Nuclear localizaition sequence

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMCMV3

Plasmid

#85711

Purpose

Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences

Depositor

Ulrich Koszinowski, Martin Messerle

Type

Empty backbone

Expression

Mammalian

Promoter

MIEPmouse CMV MIEP

Available Since

April 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.synP.DIO.EGFP.WPRE.hGH

Plasmid

#100043

Purpose

An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Ian Wickersham

Has Service

AAV9

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

synapsin-1

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pNIC28-Bsa4-TF

Plasmid

#61689

Purpose

E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted

Depositor

Brian Smith

Type

Empty backbone

Tags

HIS and Trigger Factor

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
pCS2+ H2B-Katushka2

Plasmid

#99266

Purpose

Contains a fusion of human histone H2B to fluorescent protein Katushka2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Philipp Keller

Insert

Katushka2-3XHA

Tags

H2B

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IL-2signal-6xMyc

Plasmid

#128033

Purpose

Mammalian expression vector with N terminal IL-2 signal peptide and 6xMyc tag

Depositor

Oskar Laur

Type

Empty backbone

Tags

6xMyc and IL-2 signal peptide

Expression

Mammalian

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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BII-Sh-BnTW

Plasmid

#133365

Purpose

Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS

Depositor

Blair Madison

Insert

tdTomato-NLS

Tags

Flag-tagged tdTomato

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCRII-BbsI-sgRNAscaffold

Plasmid

#159352

Purpose

Expression vector for a customizable guide RNA

Depositor

Pim Pijnappel

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pISV-DsRed1

Plasmid

#171024

Purpose

Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus.

Depositor

Christian Staehelin

Type

Empty backbone

Expression

Plant

Promoter

2X EN 35S

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-hTie1-FLAG

Plasmid

#119233

Purpose

Plasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.

Depositor

Kari Alitalo

Insert

TIE1 (TIE1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

P hUbC

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPICZa-STE2-1D4

Plasmid

#133436

Purpose

Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

Depositor

Gerhard Wagner

Insert

STE2 (STE2 Budding Yeast)

Expression

Bacterial

Mutation

C59S/C252A

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 IL-2 signal mFc

Plasmid

#128042

Purpose

Mammalian expression vector with C terminal IL-2 signal peptide and mFc

Depositor

Oskar Laur

Type

Empty backbone

Tags

IL-2 signal peptide and mFc

Expression

Mammalian

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZac-STE2-1D4

Plasmid

#133437

Purpose

Mutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

Depositor

Gerhard Wagner

Insert

STE2 (STE2 Budding Yeast)

Expression

Yeast

Mutation

C59S/C252A

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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