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pPD189 VP16-ZF37

Plasmid

#138759

Purpose

Constitutive expression of VP16-ZF37 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF37

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pKIKOrbsARKm

Plasmid

#46768

Purpose

E.coli genomic integration rbsAR locus

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pAG1242 NLS-mCherry-FIREtag

Plasmid

#205170

Purpose

Expresses NLS-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NLS-mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pE5770

Plasmid

#170777

Purpose

expression of hybrid proteins in E. coli

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

Kan resistance (kanR )

Tags

polyhisbioMbpTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

Plasmid

#175534

Purpose

Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag7-myc_PDGFRtmb

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPD543 ZF21x6-Compact_EYFP

Plasmid

#138857

Purpose

EYFP reporter vector for the ZF21x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF21x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF21x6-Compact

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD528 VP16-ZF21

Plasmid

#138757

Purpose

Constitutive expression of VP16-ZF21 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF21

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag PA1

Plasmid

#15560

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PA1 (PAGR1 Human)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-iRFP682

Plasmid

#197204

Purpose

Expresses the protein of iRFP682 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP682

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb713

Plasmid

#197207

Purpose

Expresses the protein of mRhubarb713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb713

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-BCCP-PupE

Plasmid

#113403

Purpose

Expresses BCCP-PupE in bacteria

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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6x Halo-EGFP- 2x PACT

Plasmid

#107265

Purpose

6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

6x Halo-EGFP- 2x PACT

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPD934 ZF37x6-Compact_EYFP

Plasmid

#138929

Purpose

EYFP reporter vector for the ZF37x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF37x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF37x6-Compact

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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EC59103

Plasmid

#191785

Purpose

LbCas12a expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas12a+ 8 introns-T-E9

Use

CRISPR

Expression

Plant

Mutation

D156R

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P1_AtU626Ter26

Plasmid

#191769

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-CitronRH

Plasmid

#158545

Purpose

Mammalian expression of nonbinding variant of direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

CitronRH

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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modRNAc1-Cas9-2A-puro

Plasmid

#216390

Purpose

this vector can be used to make modRNA expressing Cas9 and puro resistance gene

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

Cas9-2A-puro

Use

CRISPR

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-HisYFP-Sp100

Plasmid

#53141

Purpose

E.coli expression of HisYFP tagged human Sp100 fragment

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

Sp100A fragment (SP100 Human)

Tags

His-YFP

Expression

Bacterial

Mutation

fragment (amino acids 71-480); deletion of L75

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCGN R-Ras 43N

Plasmid

#14731

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

Insert

R-ras (RRAS Human)

Tags

HA

Expression

Mammalian

Mutation

43N

Available Since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only